Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Pancreatic cancer is a malignant tumor of the digestive tract, rendering it difficult to make an accurate diagnosis. 5-year survival rate for pancreatic cancer is <1%, and surgical resection is rarely proven to be effective. Therefore, the identification of more effective methods for early detection of pancreatic cancer is an urgent need.

This study aims to explore key genes and microRNAs (miRNAs) associated with the pathogenesis of pancreatic cancer. public databases were searched, and the integrated data from The Cancer Genome Atlas and Gene Expression Omnibus database, which leads to the identification of 23 miRNAs that are expressed differently (DE-miRNA). Four of DE-regulated miRNAs (miR-892b-HSA, HSA-miR-194-2, miR-200a-HSA and HSA-miR-194-1), while the DE-19 downregulated miRNA (hsa- Mirza 424, HSA-mir-191, mir-484-HSA, HSA-miR-142, miR-15b-HSA, HSA-miR-450A-1, HSA-mir-423, mir-126-HSA, hsa- Mirza 505, HSA-miR-16-1, HSA-miR-342, miR-130A-HSA, HSA-miR-3613, HSA-miR-450A-2, HSA-miR-26b, miR-451-HSA, HSA- miR-19b-2, HSA-miR-106A and HSA-miR-503) were identified using a cut-off criteria of P <0.05 and | log 2FC |> 1.0. Hsa-miR-3613-5p identified as prognostic DE-miRNA.

Functional enrichment analysis showed that the target gene HSA-miR-3613-5p may be related to p53 signaling pathway. survival analysis performed for the genes in the p53 signaling pathway revealed that the cyclin-dependent kinase 6 and ribonucleoside-diphosphate reductase M2 subunit might be most likely associated with prognostic value. integrated analysis performed in this study shows that the HSA-miR-3613-5p can be used as a potential prognostic marker for pancreatic cancer. Through our research, we have identified general and specific types of miRNA markers and built accurate network-based classifier miRNA, furthering our understanding of the diversity of Nen.

 Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays
Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Phylogenomic Relationships and Evolution of polyploid species Salix RAD Revealed by Sequencing Data

Polyploidy is common in the genus Salix. However, little is known about the origin, ancestry and polyploid genomes of species composition due to lack of suitable molecular markers and analysis tools.

We established a framework phylogenomic including all species of willow bushes described parts of Eurasia. We analyze the composition of the genomes of seven species of willow polyploid compared to the parental diploid species suspected to draw conclusions about their origins and effects of backcrossing and evolution of post-origin.

We applied recently developed programs such as Snapp, Hyde, and SNiPloid to build a bioinformatics pipeline to unravel the complexity of polyploid genomes. RAD sequencing revealed 23 393 320 010 SNP loci and high quality for analysis of the relationship of 35 species Eurasian shrub willow (Salix subg. Chamaetia / Vetrix). willow species seemed dominated polyploid origin allopolyploid.

sequencing system 20x50 cm

ESEQ1200-SYS ea
EUR 1842

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 174

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 262.8

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1988.4

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 781.2

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056-12 12 Reactions
EUR 625.9

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056-24 24 Reactions
EUR 1225.4

SequaGel Sequencing System 1L Kit

NAT1136 1KIT
EUR 127.68

SequaGel Sequencing System 2.2L Kit

NAT1138 EACH
EUR 217.74

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056
  • EUR 1798.26
  • EUR 625.90
  • EUR 1225.40
  • 24 Reactions
  • 12 Reactions
  • 24 Reactions

PCR Clean Up for DNA Sequencing

BT5100 100preps
EUR 114.82

PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
EUR 553.3

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 576

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

17068 5 nmoles
EUR 2091

RNU43 Primers

MPM00003 150 ul / 10 uM
EUR 145.2

snoRNA142 Primers

MPM00004 150 ul / 10 uM
EUR 145.2

U1 Primers

MP00001 150 ul / 10 uM
EUR 145.2

SNORD44 Primers

MPH00003 150 ul / 10 uM
EUR 145.2

SNORD47 Primers

MPH00004 150 ul / 10 uM
EUR 145.2

SNORD48 Primers

MPH00005 150 ul / 10 uM
EUR 145.2

Random Primers

S300 30 µg
EUR 55.2

Random Primers

S305 5x30 µg
EUR 122.4

U6 snRNA Primers

MPM00002 150 ul / 10 uM
EUR 145.2

U1 snRNA Primers

MPM00006 150 ul / 10 uM
EUR 145.2

U6-2 Primers

MPH00001 150 ul / 10 uM
EUR 145.2

rno-miR-323 Primers

MP-r00001 150 ul / 10 uM
EUR 211.2

rno-miR-301a Primers

MP-r00002 150 ul / 10 uM
EUR 211.2

rno-miR-326 Primers

MP-r00005 150 ul / 10 uM
EUR 211.2

rno-miR-327 Primers

MP-r00006 150 ul / 10 uM
EUR 211.2

rno-let-7d Primers

MP-r00007 150 ul / 10 uM
EUR 211.2

rno-miR-328 Primers

MP-r00008 150 ul / 10 uM
EUR 211.2

rno-miR-329 Primers

MP-r00009 150 ul / 10 uM
EUR 211.2

rno-miR-331 Primers

MP-r00010 150 ul / 10 uM
EUR 211.2

rno-miR-140 Primers

MP-r00011 150 ul / 10 uM
EUR 211.2

rno-miR-335 Primers

MP-r00012 150 ul / 10 uM
EUR 211.2

rno-miR-336 Primers

MP-r00013 150 ul / 10 uM
EUR 211.2

rno-miR-337 Primers

MP-r00014 150 ul / 10 uM
EUR 211.2

rno-miR-338 Primers

MP-r00015 150 ul / 10 uM
EUR 211.2

rno-miR-341 Primers

MP-r00017 150 ul / 10 uM
EUR 211.2

rno-miR-343 Primers

MP-r00018 150 ul / 10 uM
EUR 211.2

rno-miR-346 Primers

MP-r00020 150 ul / 10 uM
EUR 211.2

rno-miR-347 Primers

MP-r00021 150 ul / 10 uM
EUR 211.2

rno-miR-349 Primers

MP-r00022 150 ul / 10 uM
EUR 211.2

rno-miR-129 Primers

MP-r00023 150 ul / 10 uM
EUR 211.2

rno-miR-20a Primers

MP-r00024 150 ul / 10 uM
EUR 211.2

rno-miR-350 Primers

MP-r00025 150 ul / 10 uM
EUR 211.2

rno-miR-7a Primers

MP-r00026 150 ul / 10 uM
EUR 211.2

rno-miR-7a Primers

MP-r00027 150 ul / 10 uM
EUR 211.2

rno-miR-351 Primers

MP-r00028 150 ul / 10 uM
EUR 211.2

rno-miR-352 Primers

MP-r00029 150 ul / 10 uM
EUR 211.2

rno-miR-135b Primers

MP-r00030 150 ul / 10 uM
EUR 211.2

rno-miR-151 Primers

MP-r00031 150 ul / 10 uM
EUR 211.2

rno-miR-101b Primers

MP-r00032 150 ul / 10 uM
EUR 211.2

rno-let-7a Primers

MP-r00033 150 ul / 10 uM
EUR 211.2

rno-let-7b Primers

MP-r00034 150 ul / 10 uM
EUR 211.2

rno-let-7c Primers

MP-r00035 150 ul / 10 uM
EUR 211.2

rno-let-7e Primers

MP-r00036 150 ul / 10 uM
EUR 211.2

rno-let-7f Primers

MP-r00037 150 ul / 10 uM
EUR 211.2

rno-let-7i Primers

MP-r00038 150 ul / 10 uM
EUR 211.2

rno-miR-7b Primers

MP-r00039 150 ul / 10 uM
EUR 211.2

rno-miR-9 Primers

MP-r00040 150 ul / 10 uM
EUR 211.2

rno-miR-10b Primers

MP-r00042 150 ul / 10 uM
EUR 211.2

rno-miR-15b Primers

MP-r00043 150 ul / 10 uM
EUR 211.2

rno-miR-16 Primers

MP-r00044 150 ul / 10 uM
EUR 211.2

rno-miR-18a Primers

MP-r00047 150 ul / 10 uM
EUR 211.2

rno-miR-19b Primers

MP-r00048 150 ul / 10 uM
EUR 211.2

rno-miR-19a Primers

MP-r00049 150 ul / 10 uM
EUR 211.2

rno-miR-21 Primers

MP-r00050 150 ul / 10 uM
EUR 211.2

rno-miR-22 Primers

MP-r00051 150 ul / 10 uM
EUR 211.2

rno-miR-23a Primers

MP-r00052 150 ul / 10 uM
EUR 211.2

rno-miR-23b Primers

MP-r00053 150 ul / 10 uM
EUR 211.2

rno-miR-24 Primers

MP-r00054 150 ul / 10 uM
EUR 211.2

rno-miR-24 Primers

MP-r00055 150 ul / 10 uM
EUR 211.2

rno-miR-25 Primers

MP-r00056 150 ul / 10 uM
EUR 211.2

rno-miR-26a Primers

MP-r00057 150 ul / 10 uM
EUR 211.2

rno-miR-26b Primers

MP-r00058 150 ul / 10 uM
EUR 211.2

rno-miR-27b Primers

MP-r00059 150 ul / 10 uM
EUR 211.2

rno-miR-27a Primers

MP-r00060 150 ul / 10 uM
EUR 211.2

rno-miR-28 Primers

MP-r00061 150 ul / 10 uM
EUR 211.2

rno-miR-29b Primers

MP-r00062 150 ul / 10 uM
EUR 211.2

rno-miR-29b Primers

MP-r00063 150 ul / 10 uM
EUR 211.2

rno-miR-29a Primers

MP-r00064 150 ul / 10 uM
EUR 211.2

rno-miR-29c Primers

MP-r00065 150 ul / 10 uM
EUR 211.2

rno-miR-30c Primers

MP-r00066 150 ul / 10 uM
EUR 211.2

rno-miR-30e Primers

MP-r00067 150 ul / 10 uM
EUR 211.2

rno-miR-30d Primers

MP-r00069 150 ul / 10 uM
EUR 211.2

rno-miR-30a Primers

MP-r00070 150 ul / 10 uM
EUR 211.2

rno-miR-31 Primers

MP-r00071 150 ul / 10 uM
EUR 211.2

rno-miR-32 Primers

MP-r00072 150 ul / 10 uM
EUR 211.2

rno-miR-33 Primers

MP-r00073 150 ul / 10 uM
EUR 211.2

rno-miR-34b Primers

MP-r00074 150 ul / 10 uM
EUR 211.2

rno-miR-34c Primers

MP-r00075 150 ul / 10 uM
EUR 211.2

rno-miR-34a Primers

MP-r00076 150 ul / 10 uM
EUR 211.2

rno-miR-92a Primers

MP-r00077 150 ul / 10 uM
EUR 211.2

rno-miR-93 Primers

MP-r00078 150 ul / 10 uM
EUR 211.2

rno-miR-96 Primers

MP-r00079 150 ul / 10 uM
EUR 211.2

rno-miR-98 Primers

MP-r00080 150 ul / 10 uM
EUR 211.2

rno-miR-99a Primers

MP-r00081 150 ul / 10 uM
EUR 211.2

rno-miR-99b Primers

MP-r00082 150 ul / 10 uM
EUR 211.2

rno-miR-100 Primers

MP-r00083 150 ul / 10 uM
EUR 211.2

rno-miR-101a Primers

MP-r00084 150 ul / 10 uM
EUR 211.2

hsa-miR-4672 Primers

MPH02854 150 ul / 10 uM
EUR 145.2

hsa-miR-4673 Primers

MPH02855 150 ul / 10 uM
EUR 145.2

hsa-miR-4674 Primers

MPH02856 150 ul / 10 uM
EUR 145.2

hsa-miR-4675 Primers

MPH02857 150 ul / 10 uM
EUR 145.2

hsa-miR-4678 Primers

MPH02862 150 ul / 10 uM
EUR 145.2

hsa-miR-4679 Primers

MPH02863 150 ul / 10 uM
EUR 145.2

hsa-miR-4681 Primers

MPH02866 150 ul / 10 uM
EUR 145.2

hsa-miR-4682 Primers

MPH02867 150 ul / 10 uM
EUR 145.2

hsa-miR-4683 Primers

MPH02868 150 ul / 10 uM
EUR 145.2

hsa-miR-4686 Primers

MPH02873 150 ul / 10 uM
EUR 145.2

hsa-miR-4688 Primers

MPH02876 150 ul / 10 uM
EUR 145.2

hsa-miR-4689 Primers

MPH02877 150 ul / 10 uM
EUR 145.2

hsa-miR-4692 Primers

MPH02882 150 ul / 10 uM
EUR 145.2

hsa-miR-4696 Primers

MPH02889 150 ul / 10 uM
EUR 145.2

hsa-miR-4698 Primers

MPH02892 150 ul / 10 uM
EUR 145.2

hsa-miR-4705 Primers

MPH02903 150 ul / 10 uM
EUR 145.2

hsa-miR-4706 Primers

MPH02904 150 ul / 10 uM
EUR 145.2

hsa-miR-4710 Primers

MPH02911 150 ul / 10 uM
EUR 145.2

hsa-miR-4718 Primers

MPH02926 150 ul / 10 uM
EUR 145.2

hsa-miR-4719 Primers

MPH02927 150 ul / 10 uM
EUR 145.2

hsa-miR-4721 Primers

MPH02930 150 ul / 10 uM
EUR 145.2

hsa-miR-4729 Primers

MPH02945 150 ul / 10 uM
EUR 145.2

hsa-miR-4730 Primers

MPH02946 150 ul / 10 uM
EUR 145.2

hsa-miR-4734 Primers

MPH02953 150 ul / 10 uM
EUR 145.2

hsa-miR-4736 Primers

MPH02956 150 ul / 10 uM
EUR 145.2

hsa-miR-4737 Primers

MPH02957 150 ul / 10 uM
EUR 145.2

hsa-miR-4739 Primers

MPH02960 150 ul / 10 uM
EUR 145.2

hsa-miR-4741 Primers

MPH02963 150 ul / 10 uM
EUR 145.2

hsa-miR-4744 Primers

MPH02968 150 ul / 10 uM
EUR 145.2

hsa-miR-4748 Primers

MPH02975 150 ul / 10 uM
EUR 145.2

hsa-miR-4751 Primers

MPH02980 150 ul / 10 uM
EUR 145.2

hsa-miR-4752 Primers

MPH02981 150 ul / 10 uM
EUR 145.2

hsa-miR-4754 Primers

MPH02984 150 ul / 10 uM
EUR 145.2

hsa-miR-4759 Primers

MPH02993 150 ul / 10 uM
EUR 145.2

hsa-miR-4765 Primers

MPH03004 150 ul / 10 uM
EUR 145.2

hsa-miR-4767 Primers

MPH03007 150 ul / 10 uM
EUR 145.2

hsa-miR-4770 Primers

MPH03012 150 ul / 10 uM
EUR 145.2

hsa-miR-4771 Primers

MPH03013 150 ul / 10 uM
EUR 145.2

hsa-miR-4773 Primers

MPH03016 150 ul / 10 uM
EUR 145.2

hsa-miR-4775 Primers

MPH03019 150 ul / 10 uM
EUR 145.2

hsa-miR-4779 Primers

MPH03026 150 ul / 10 uM
EUR 145.2

hsa-miR-4780 Primers

MPH03027 150 ul / 10 uM
EUR 145.2

hsa-miR-4784 Primers

MPH03034 150 ul / 10 uM
EUR 145.2

hsa-miR-4785 Primers

MPH03035 150 ul / 10 uM
EUR 145.2

hsa-miR-4788 Primers

MPH03040 150 ul / 10 uM
EUR 145.2

hsa-miR-4791 Primers

MPH03045 150 ul / 10 uM
EUR 145.2

hsa-miR-4792 Primers

MPH03046 150 ul / 10 uM
EUR 145.2

hsa-miR-4794 Primers

MPH03049 150 ul / 10 uM
EUR 145.2

hsa-miR-4801 Primers

MPH03062 150 ul / 10 uM
EUR 145.2

hsa-miR-4803 Primers

MPH03065 150 ul / 10 uM
EUR 145.2

hsa-miR-5047 Primers

MPH03119 150 ul / 10 uM
EUR 145.2

hsa-miR-5087 Primers

MPH03124 150 ul / 10 uM
EUR 145.2

hsa-miR-5089 Primers

MPH03127 150 ul / 10 uM
EUR 145.2

hsa-miR-5090 Primers

MPH03129 150 ul / 10 uM
EUR 145.2

hsa-miR-5091 Primers

MPH03130 150 ul / 10 uM
EUR 145.2

hsa-miR-5092 Primers

MPH03131 150 ul / 10 uM
EUR 145.2

hsa-miR-5093 Primers

MPH03132 150 ul / 10 uM
EUR 145.2

hsa-miR-5094 Primers

MPH03133 150 ul / 10 uM
EUR 145.2

hsa-miR-5095 Primers

MPH03134 150 ul / 10 uM
EUR 145.2

Allopolyploidization more ancient events were observed during the two hexaploid and one species octoploid, whereas our data suggest more recent origin for tetraploids allopolyploid including parental taxa and identifying suspected. SNiPloid analysis parse different genomic signatures generated from hybrid origin, backcrossing, and the evolution of post-secondary origin in polyploid species. Our RAD sequencing data indicate that willow genome evolution shaped by ancient and recently reticulate, polyploidization, and post-native species differences.