Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Pancreatic cancer is a malignant tumor of the digestive tract, rendering it difficult to make an accurate diagnosis. 5-year survival rate for pancreatic cancer is <1%, and surgical resection is rarely proven to be effective. Therefore, the identification of more effective methods for early detection of pancreatic cancer is an urgent need.

This study aims to explore key genes and microRNAs (miRNAs) associated with the pathogenesis of pancreatic cancer. public databases were searched, and the integrated data from The Cancer Genome Atlas and Gene Expression Omnibus database, which leads to the identification of 23 miRNAs that are expressed differently (DE-miRNA). Four of DE-regulated miRNAs (miR-892b-HSA, HSA-miR-194-2, miR-200a-HSA and HSA-miR-194-1), while the DE-19 downregulated miRNA (hsa- Mirza 424, HSA-mir-191, mir-484-HSA, HSA-miR-142, miR-15b-HSA, HSA-miR-450A-1, HSA-mir-423, mir-126-HSA, hsa- Mirza 505, HSA-miR-16-1, HSA-miR-342, miR-130A-HSA, HSA-miR-3613, HSA-miR-450A-2, HSA-miR-26b, miR-451-HSA, HSA- miR-19b-2, HSA-miR-106A and HSA-miR-503) were identified using a cut-off criteria of P <0.05 and | log 2FC |> 1.0. Hsa-miR-3613-5p identified as prognostic DE-miRNA.

Functional enrichment analysis showed that the target gene HSA-miR-3613-5p may be related to p53 signaling pathway. survival analysis performed for the genes in the p53 signaling pathway revealed that the cyclin-dependent kinase 6 and ribonucleoside-diphosphate reductase M2 subunit might be most likely associated with prognostic value. integrated analysis performed in this study shows that the HSA-miR-3613-5p can be used as a potential prognostic marker for pancreatic cancer. Through our research, we have identified general and specific types of miRNA markers and built accurate network-based classifier miRNA, furthering our understanding of the diversity of Nen.

 Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays
Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Phylogenomic Relationships and Evolution of polyploid species Salix RAD Revealed by Sequencing Data

Polyploidy is common in the genus Salix. However, little is known about the origin, ancestry and polyploid genomes of species composition due to lack of suitable molecular markers and analysis tools.

We established a framework phylogenomic including all species of willow bushes described parts of Eurasia. We analyze the composition of the genomes of seven species of willow polyploid compared to the parental diploid species suspected to draw conclusions about their origins and effects of backcrossing and evolution of post-origin.

We applied recently developed programs such as Snapp, Hyde, and SNiPloid to build a bioinformatics pipeline to unravel the complexity of polyploid genomes. RAD sequencing revealed 23 393 320 010 SNP loci and high quality for analysis of the relationship of 35 species Eurasian shrub willow (Salix subg. Chamaetia / Vetrix). willow species seemed dominated polyploid origin allopolyploid.

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1657

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 651

Chymotrypsin for Sequencing grade

C4001-010 4x25ug
EUR 313

Chymotrypsin for Sequencing grade

C4001-100 100ug
EUR 286

SequaGel Sequencing System 1L Kit

NAT1136 1KIT
EUR 143

SequaGel Sequencing System 2.2L Kit

NAT1138 EACH
EUR 211

PCR Clean Up for DNA Sequencing

BT5100 100preps
EUR 95.68

PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
EUR 461.08

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 480

Random Primers

S300 30 µg
EUR 46

Random Primers

S305 5x30 µg
EUR 102

U1 Primers

MP00001 150 ul / 10 uM
EUR 121

SNORD44 Primers

MPH00003 150 ul / 10 uM
EUR 121

SNORD47 Primers

MPH00004 150 ul / 10 uM
EUR 121

SNORD48 Primers

MPH00005 150 ul / 10 uM
EUR 121

RNU43 Primers

MPM00003 150 ul / 10 uM
EUR 121

snoRNA142 Primers

MPM00004 150 ul / 10 uM
EUR 121

U6-2 Primers

MPH00001 150 ul / 10 uM
EUR 121

U6 snRNA Primers

MPM00002 150 ul / 10 uM
EUR 121

U1 snRNA Primers

MPM00006 150 ul / 10 uM
EUR 121

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 10/pk
EUR 533
Description: Sealing Products; Sealing mats - Axygen

mmu-miR-1190 Primers

MPM00030 150 ul / 10 uM
EUR 121

mmu-miR-1191 Primers

MPM00031 150 ul / 10 uM
EUR 121

mmu-miR-1192 Primers

MPM00032 150 ul / 10 uM
EUR 121

mmu-miR-1194 Primers

MPM00035 150 ul / 10 uM
EUR 121

mmu-miR-1195 Primers

MPM00036 150 ul / 10 uM
EUR 121

mmu-miR-1196 Primers

MPM00037 150 ul / 10 uM
EUR 121

mmu-miR-1197 Primers

MPM00038 150 ul / 10 uM
EUR 121

mmu-miR-1199 Primers

MPM00041 150 ul / 10 uM
EUR 121

mmu-miR-122 Primers

MPM00042 150 ul / 10 uM
EUR 121

mmu-miR-1224 Primers

MPM00043 150 ul / 10 uM
EUR 121

mmu-miR-124 Primers

MPM00044 150 ul / 10 uM
EUR 121

mmu-miR-1247 Primers

MPM00045 150 ul / 10 uM
EUR 121

mmu-miR-1249 Primers

MPM00046 150 ul / 10 uM
EUR 121

mmu-miR-1251 Primers

MPM00047 150 ul / 10 uM
EUR 121

mmu-miR-127 Primers

MPM00057 150 ul / 10 uM
EUR 121

mmu-miR-1274a Primers

MPM00058 150 ul / 10 uM
EUR 121

mmu-miR-128 Primers

MPM00059 150 ul / 10 uM
EUR 121

mmu-miR-1298 Primers

MPM00064 150 ul / 10 uM
EUR 121

mmu-miR-1306 Primers

MPM00067 150 ul / 10 uM
EUR 121

mmu-miR-130a Primers

MPM00068 150 ul / 10 uM
EUR 121

mmu-miR-130b Primers

MPM00069 150 ul / 10 uM
EUR 121

mmu-miR-132 Primers

MPM00070 150 ul / 10 uM
EUR 121

mmu-miR-133a Primers

MPM00071 150 ul / 10 uM
EUR 121

mmu-miR-133b Primers

MPM00072 150 ul / 10 uM
EUR 121

mmu-miR-134 Primers

MPM00073 150 ul / 10 uM
EUR 121

mmu-miR-135a Primers

MPM00074 150 ul / 10 uM
EUR 121

mmu-miR-135b Primers

MPM00075 150 ul / 10 uM
EUR 121

mmu-miR-136 Primers

MPM00076 150 ul / 10 uM
EUR 121

mmu-miR-137 Primers

MPM00077 150 ul / 10 uM
EUR 121

mmu-miR-138 Primers

MPM00078 150 ul / 10 uM
EUR 121

mmu-miR-140 Primers

MPM00081 150 ul / 10 uM
EUR 121

mmu-miR-140* Primers

MPM00082 150 ul / 10 uM
EUR 121

mmu-miR-141 Primers

MPM00083 150 ul / 10 uM
EUR 121

mmu-miR-143 Primers

MPM00086 150 ul / 10 uM
EUR 121

mmu-miR-144 Primers

MPM00087 150 ul / 10 uM
EUR 121

mmu-miR-145 Primers

MPM00088 150 ul / 10 uM
EUR 121

mmu-miR-146a Primers

MPM00089 150 ul / 10 uM
EUR 121

mmu-miR-146b Primers

MPM00090 150 ul / 10 uM
EUR 121

mmu-miR-147 Primers

MPM00091 150 ul / 10 uM
EUR 121

mmu-miR-148a Primers

MPM00092 150 ul / 10 uM
EUR 121

mmu-miR-148b Primers

MPM00093 150 ul / 10 uM
EUR 121

mmu-miR-149 Primers

MPM00094 150 ul / 10 uM
EUR 121

mmu-miR-150 Primers

MPM00095 150 ul / 10 uM
EUR 121

mmu-miR-152 Primers

MPM00098 150 ul / 10 uM
EUR 121

mmu-miR-153 Primers

MPM00099 150 ul / 10 uM
EUR 121

mmu-miR-154 Primers

MPM00100 150 ul / 10 uM
EUR 121

mmu-miR-155 Primers

MPM00101 150 ul / 10 uM
EUR 121

mmu-miR-15a Primers

MPM00102 150 ul / 10 uM
EUR 121

mmu-miR-15b Primers

MPM00103 150 ul / 10 uM
EUR 121

mmu-miR-16 Primers

MPM00104 150 ul / 10 uM
EUR 121

mmu-miR-17 Primers

MPM00105 150 ul / 10 uM
EUR 121

mmu-miR-181a Primers

MPM00106 150 ul / 10 uM
EUR 121

mmu-miR-181b Primers

MPM00107 150 ul / 10 uM
EUR 121

mmu-miR-181c Primers

MPM00108 150 ul / 10 uM
EUR 121

mmu-miR-181d Primers

MPM00109 150 ul / 10 uM
EUR 121

mmu-miR-182 Primers

MPM00110 150 ul / 10 uM
EUR 121

mmu-miR-183 Primers

MPM00111 150 ul / 10 uM
EUR 121

mmu-miR-184 Primers

MPM00114 150 ul / 10 uM
EUR 121

mmu-miR-185 Primers

MPM00117 150 ul / 10 uM
EUR 121

mmu-miR-186 Primers

MPM00118 150 ul / 10 uM
EUR 121

mmu-miR-187 Primers

MPM00119 150 ul / 10 uM
EUR 121

mmu-miR-1892 Primers

MPM00122 150 ul / 10 uM
EUR 121

mmu-miR-1893 Primers

MPM00123 150 ul / 10 uM
EUR 121

mmu-miR-1895 Primers

MPM00126 150 ul / 10 uM
EUR 121

mmu-miR-1896 Primers

MPM00127 150 ul / 10 uM
EUR 121

mmu-miR-1898 Primers

MPM00130 150 ul / 10 uM
EUR 121

mmu-miR-1899 Primers

MPM00131 150 ul / 10 uM
EUR 121

mmu-miR-18a Primers

MPM00132 150 ul / 10 uM
EUR 121

mmu-miR-18b Primers

MPM00133 150 ul / 10 uM
EUR 121

mmu-miR-190 Primers

MPM00134 150 ul / 10 uM
EUR 121

mmu-miR-1900 Primers

MPM00135 150 ul / 10 uM
EUR 121

mmu-miR-1901 Primers

MPM00136 150 ul / 10 uM
EUR 121

mmu-miR-1902 Primers

MPM00137 150 ul / 10 uM
EUR 121

mmu-miR-1903 Primers

MPM00138 150 ul / 10 uM
EUR 121

mmu-miR-1904 Primers

MPM00139 150 ul / 10 uM
EUR 121

Allopolyploidization more ancient events were observed during the two hexaploid and one species octoploid, whereas our data suggest more recent origin for tetraploids allopolyploid including parental taxa and identifying suspected. SNiPloid analysis parse different genomic signatures generated from hybrid origin, backcrossing, and the evolution of post-secondary origin in polyploid species. Our RAD sequencing data indicate that willow genome evolution shaped by ancient and recently reticulate, polyploidization, and post-native species differences.