Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Pancreatic cancer is a malignant tumor of the digestive tract, rendering it difficult to make an accurate diagnosis. 5-year survival rate for pancreatic cancer is <1%, and surgical resection is rarely proven to be effective. Therefore, the identification of more effective methods for early detection of pancreatic cancer is an urgent need.

This study aims to explore key genes and microRNAs (miRNAs) associated with the pathogenesis of pancreatic cancer. public databases were searched, and the integrated data from The Cancer Genome Atlas and Gene Expression Omnibus database, which leads to the identification of 23 miRNAs that are expressed differently (DE-miRNA). Four of DE-regulated miRNAs (miR-892b-HSA, HSA-miR-194-2, miR-200a-HSA and HSA-miR-194-1), while the DE-19 downregulated miRNA (hsa- Mirza 424, HSA-mir-191, mir-484-HSA, HSA-miR-142, miR-15b-HSA, HSA-miR-450A-1, HSA-mir-423, mir-126-HSA, hsa- Mirza 505, HSA-miR-16-1, HSA-miR-342, miR-130A-HSA, HSA-miR-3613, HSA-miR-450A-2, HSA-miR-26b, miR-451-HSA, HSA- miR-19b-2, HSA-miR-106A and HSA-miR-503) were identified using a cut-off criteria of P <0.05 and | log 2FC |> 1.0. Hsa-miR-3613-5p identified as prognostic DE-miRNA.

Functional enrichment analysis showed that the target gene HSA-miR-3613-5p may be related to p53 signaling pathway. survival analysis performed for the genes in the p53 signaling pathway revealed that the cyclin-dependent kinase 6 and ribonucleoside-diphosphate reductase M2 subunit might be most likely associated with prognostic value. integrated analysis performed in this study shows that the HSA-miR-3613-5p can be used as a potential prognostic marker for pancreatic cancer. Through our research, we have identified general and specific types of miRNA markers and built accurate network-based classifier miRNA, furthering our understanding of the diversity of Nen.

 Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays
Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Phylogenomic Relationships and Evolution of polyploid species Salix RAD Revealed by Sequencing Data

Polyploidy is common in the genus Salix. However, little is known about the origin, ancestry and polyploid genomes of species composition due to lack of suitable molecular markers and analysis tools.

We established a framework phylogenomic including all species of willow bushes described parts of Eurasia. We analyze the composition of the genomes of seven species of willow polyploid compared to the parental diploid species suspected to draw conclusions about their origins and effects of backcrossing and evolution of post-origin.

We applied recently developed programs such as Snapp, Hyde, and SNiPloid to build a bioinformatics pipeline to unravel the complexity of polyploid genomes. RAD sequencing revealed 23 393 320 010 SNP loci and high quality for analysis of the relationship of 35 species Eurasian shrub willow (Salix subg. Chamaetia / Vetrix). willow species seemed dominated polyploid origin allopolyploid.

sequencing system 20x50 cm

ESEQ1200-SYS ea
EUR 1842

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 174

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 262.8

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1988.4

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 781.2

Sequencing Grade V8 Protease

RE014 50ug
EUR 209

Chymotrypsin for Sequencing grade

C4001-010 4x25ug
EUR 375.6

Chymotrypsin for Sequencing grade

C4001-100 100ug
EUR 343.2

Sequencing Grade Modified Trypsin

RE012 100ug
EUR 229

PCR Clean Up for DNA Sequencing

BT5100 100preps
EUR 114.82

PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
EUR 553.3

Sequencing Grade Carboxypeptidase B

RE011 100ug
EUR 99

ACES Sequencing Buffer 10X, pH 7.7

21420000-1 1 L
EUR 138.62

ACES Sequencing Buffer 10X, pH 7.7

21420000-2 4 L
EUR 456.17

TBE-Buffer 5X for DNA Sequencing

40121072-1 100 mL
EUR 26.54

TBE-Buffer 5X for DNA Sequencing

40121072-2 500 mL
EUR 44.84

TBE-Buffer 5X for DNA Sequencing

40121072-3 1 L
EUR 72.29

TBE-Buffer 5X for DNA Sequencing

40121072-4 4 L
EUR 192.14

SequaGel Sequencing System 1L Kit - 1KIT

NAT1136 1KIT
EUR 187.65

SequaGel Sequencing System 2.2L Kit - EACH

NAT1138 EACH
EUR 318.6

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ100UG each
EUR 148
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ1MG each
EUR 765
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ400UG each
EUR 419
Description: Bovine Trypsin Purified Sequencing Grade

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056
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  • Ask for price
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  • 24 Reactions
  • 12 Reactions
  • 24 Reactions

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 576

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY100UG each
EUR 130
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY1MG each
EUR 640
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY400UG each
EUR 344
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

17068 5 nmoles
EUR 2091

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

IBOCHYTLCKSG100UG each
EUR 297
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

iLenti Sequence Primers

LV012 100 μl (10 μM)
EUR 75

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

IBCTCLOSGPF10UG each
EUR 165
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY250UG each
EUR 784
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY50UG each
EUR 304
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 10/pk
EUR 639.6
Description: Sealing Products; Sealing mats - Axygen

Human Stem Cell Primer Library

HSCL-I 1 set
EUR 540

Rat NFKappaB Primer Library

RNFKB-I 1 set
EUR 774

Rat Cytokine Primer Library I

RCA-I 1 set
EUR 657.6

Rat Cytokine Primer Library II

RCA-II 1 set
EUR 657.6

Human DNA Repair Primer Library I

HDRL-I 1 set
EUR 657.6

Human DNA Repair Primer Library II

HDRL-II 1 set
EUR 657.6

Human Cell Cycle Primer Library

HCC-I 1 set
EUR 540

Rat Autophagy Primer Library

RATPL-I 1 set
EUR 774

Mouse DNA Repair Primer Library I

MDRL-I 1 set
EUR 774

Mouse DNA Repair Primer Library II

MDRL-II 1 set
EUR 774

Mouse Cell Cycle Primer Library

MCC-I 1 set
EUR 540

Human Cytokine Primer Library I

HCA-I 1 set
EUR 540

Human Cytokine Primer Library II

HCA-II 1 set
EUR 540

Mouse Oncogene Primer Library

MONC-I 1 set
EUR 657.6

Mouse NFKappaB Primer Library

MNFKB-I 1 set
EUR 540

Human Melanoma Primer Library

HMEL-I 1 set
EUR 657.6

Human NFKappaB Primer Library

HNFKB-I 1 set
EUR 540

Human Oncogene Primer Library

HONC-I 1 set
EUR 657.6

Mouse Cytokine Primer Library I

MCA-I 1 set
EUR 540

Mouse Cytokine Primer Library II

MCA-II 1 set
EUR 540

Mouse Autophagy Primer Library

MATPL-I 1 set
EUR 540

Human Autophagy Primer Library

HATPL-I 1 set
EUR 540

Human Brain Cancer Primer Library

HBRC-I 1 set
EUR 657.6

Human Colon Cancer Primer Library I

HCCP-I 1 set
EUR 657.6

Human Colon Cancer Primer Library II

HCCP-II 1 set
EUR 657.6

Mouse Apoptosis Primer Library

MPA-I 1 set
EUR 540

Rat Gdnf Signaling Primer Library

RGDNF-I 1 set
EUR 774

Human Lung Cancer PCR Primer Library

HLUCPL-I 1 set
EUR 657.6

Human Apoptosis Primer Library

HPA-I 1 set
EUR 540

Human Liver Cancer Primer Library

HLCPL-I 1 set
EUR 657.6

Rat Bdnf Signaling Primer Library

RBDNF-I 1 set
EUR 774

Mouse BMP Signaling Primer Library

MBMP-I 1 set
EUR 657.6

Human BMP Signaling Primer Library

HBMP-I 1 set
EUR 657.6

Human Cell Adhesion Primer Library

HCAD-I 1 set
EUR 774

Human WNT Signaling Primer Library

HWNT-I 1 set
EUR 657.6

Human Homeobox Gene Primer Library

HHOX-I 1 set
EUR 774

Human Ras Signaling Primer Library

HRAS-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library

HSCH-I 1 set
EUR 657.6

Human TNF Signaling Primer Library

HTNF-I 1 set
EUR 657.6

Human p53 Signaling Primer Library

HTPS-I 1 set
EUR 540

Mouse Ras Signaling Primer Library

MRAS-I 1 set
EUR 657.6

Mouse p53 Signaling Primer Library

MTPS-I 1 set
EUR 540

Mouse WNT Signaling Primer Library

MWNT-I 1 set
EUR 657.6

Mouse Bdnf Signaling Primer Library

MBDNF-I 1 set
EUR 657.6

Human EGFR Signaling Primer Library

HEGFR-I 1 set
EUR 657.6

Human ERBB Signaling Primer Library

HERBB-I 1 set
EUR 657.6

Human BDNF Signaling Primer Library

HBDNF-I 1 set
EUR 657.6

Human Bladder Cancer Primer Library

HBLCP-I 1 set
EUR 657.6

Human cAMP Signaling Primer Library

HCAMP-I 1 set
EUR 657.6

Human Cancer Pathway Primer Library

HCAN-I 1 set
EUR 657.6

Human CREB Signaling Primer Library

HCREB-I 1 set
EUR 657.6

Human VEGF Signaling Primer Library

HVEGF-I 1 set
EUR 657.6

Human Necroptosis Primer Library

HNCR-I 1 set
EUR 657.6

Human Ovarian Cancer Primer Library

HOVCL-I 1 set
EUR 657.6

Human PDGF Signaling Primer Library

HPDGF-I 1 set
EUR 657.6

Human Gastric Cancer Primer Library

HGCPL-I 1 set
EUR 657.6

Human GDNF Signaling Primer Library

HGDNF-I 1 set
EUR 657.6

Human IL17 Signaling Primer Library

HIL17-I 1 set
EUR 657.6

Human SMAD Signaling Primer Library

HSMAD-I 1 set
EUR 657.6

Human Tumor Immunity Primer Library

HTIMU-I 1 set
EUR 657.6

Mouse EGFR Signaling Primer Library

MEGFR-I 1 set
EUR 657.6

Mouse CREB Signaling Primer Library

MCREB-I 1 set
EUR 657.6

Mouse Gdnf Signaling Primer Library

MGDNF-I 1 set
EUR 657.6

Mouse Tumor Immunity Primer Library

MTIMU-I 1 set
EUR 657.6

Rat Oxidative Stress Primer Library

ROSL-I 1 set
EUR 657.6

Mouse Angiogenesis Primer Library

MAPL-I 1 set
EUR 540

Mouse Adipogenesis Primer Library

MADG-I 1 set
EUR 657.6

Human Adipogenesis Primer Library

HADG-I 1 set
EUR 657.6

Human Angiogenesis Primer Library

HAPL-I 1 set
EUR 540

Human Cancer Driver Gene I Primer Library

HCDG-I 1 set
EUR 774

Human Cancer Driver Gene II Primer Library

HCDG-II 1 set
EUR 774

Mouse Notch Signaling Primer Library

MNOT-I 1 set
EUR 657.6

Mouse Jak/Stat Signaling Primer Library

MJAK-I 1 set
EUR 774

Human Osteogenesis Primer Library

HOST-I 1 set
EUR 657.6

Human Prostate Cancer Primer Library

HPRC-I 1 set
EUR 657.6

Human Hippo Signaling Primer Pathway

HHPO-I 1 set
EUR 657.6

Human Heat Shock Protein Primer Library

HHSP-I 1 set
EUR 891.6

Human JAK/STAT Signaling Primer Library

HJAK-I 1 set
EUR 657.6

Human Cytoskeleton Primer Library

HSCK-I 1 set
EUR 774

Human TGF Beta Signaling Primer Library

HTGFB-I 1 set
EUR 657.6

Mouse Hippo Signaling Primer Library

MHPO-I 1 set
EUR 657.6

Mouse TGF Beta Signaling Primer Library

MTGFB-I 1 set
EUR 657.6

Human Drug Transporter I Primer Library

HDTP-I 1 set
EUR 657.6

Human Endothelial Cell Primer Library

HEND-I 1 set
EUR 657.6

Mouse Oxidative Stress Primer Library

MOSL-I 1 set
EUR 657.6

Rat Glucose Metabolism Primer Library

RGLM-I 1 set
EUR 657.6

Rat Integrin Signaling Primer Library

RINT-I 1 set
EUR 1008

Human Oxidative Stress Primer Library

HOSL-I 1 set
EUR 540

Mouse PI3K-AKT Signaling Primer Library

MAKT-I 1 set
EUR 657.6

Human PI3K-AKT Signaling Primer Library

HAKT-I 1 set
EUR 540

Human Alzheimer's Disease Primer Library

HAZD-I 1 set
EUR 657.6

Mouse MAP Kinase Signaling Primer Library

MMAP-I 1 set
EUR 657.6

Human Pancreatic Cancer Primer Library

HPANC-I 1 set
EUR 657.6

Human Parkinson's Disease Primer Library

HPKD-I 1 set
EUR 657.6

Human Hypoxia Signaling Primer Library

HHYP-I 1 set
EUR 657.6

Human Insulin Signaling Primer Library

HINS-I 1 set
EUR 657.6

Mouse DNA Damage Signaling Primer Library

MDDS-I 1 set
EUR 657.6

Mouse Hypoxia Signaling Primer Library

MHYP-I 1 set
EUR 657.6

Human Estrogen Signaling Primer Library

HESR-I 1 set
EUR 774

Human Amino Acid Metabolism Primer Library

HAMM-I 1 set
EUR 774

Human Androgen Signaling Primer Library

HAND-I 1 set
EUR 657.6

Human Atherosclerosis Primer Library

HATH-I 1 set
EUR 657.6

Mouse Integrin Signaling Primer Library

MINT-I 1 set
EUR 891.6

Rat Ubiquitin Metabolism Primer Library

RUBM-I 1 set
EUR 774

Human Glucose Metabolism Primer Library

HGLM-I 1 set
EUR 540

Human Hedgehog Signaling Primer Library

HHOG-I 1 set
EUR 774

Human Integrin Signaling Primer Library

HINT-I 1 set
EUR 657.6

Mouse Fatty Liver Signaling Primer Library

MFTL-I 1 set
EUR 657.6

Mouse Glucose Metabolism Primer Library

MGLM-I 1 set
EUR 657.6

Mouse Adherin - Tight Junction Primer Library

MATJ-I 1 set
EUR 657.6

Human Adherin - Tight Junction Primer Library

HATJ-I 1 set
EUR 657.6

Human NK Cell Mediated Toxicity Primer Library

HNKT-I 1 set
EUR 657.6

Human Nitric Oxide Signaling Primer Library

HNOS-I 1 set
EUR 657.6

Human FGF Receptor Signaling Primer Library

HFGF-I 1 set
EUR 774

Human Ligand Gated Ion Channels Primer Library

HLGI-I 1 set
EUR 891.6

Human Retinoblastoma Gene Primer Library

HRBG-I 1 set
EUR 657.6

Human Rho GTPase-Signaling Primer Library

HRGTP-I 1 set
EUR 774

Human Signal Transduction Primer Library

HSTL-I 1 set
EUR 540

Human Breast Cancer Signaling Primer Library

HBRCA-I 1 set
EUR 657.6

Human Chromatin Regulation Primer Library

HCHR-I 1 set
EUR 774

Human B-Cell Receptor Signaling Primer Library

HBCR-I 1 set
EUR 657.6

Human Focal Adhesion Signaling Primer Library

HFCA-I 1 set
EUR 657.6

Human T-Cell Receptor Signaling Primer Library

HTCR-I 1 set
EUR 657.6

Mouse T-Cell Receptor Signaling Primer Library

MTCR-I 1 set
EUR 657.6

Human Fatty Acid and Lipid Metabolism Primer Library

HFLM-I 1 set
EUR 657.6

Allopolyploidization more ancient events were observed during the two hexaploid and one species octoploid, whereas our data suggest more recent origin for tetraploids allopolyploid including parental taxa and identifying suspected. SNiPloid analysis parse different genomic signatures generated from hybrid origin, backcrossing, and the evolution of post-secondary origin in polyploid species. Our RAD sequencing data indicate that willow genome evolution shaped by ancient and recently reticulate, polyploidization, and post-native species differences.