Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Pancreatic cancer is a malignant tumor of the digestive tract, rendering it difficult to make an accurate diagnosis. 5-year survival rate for pancreatic cancer is <1%, and surgical resection is rarely proven to be effective. Therefore, the identification of more effective methods for early detection of pancreatic cancer is an urgent need.

This study aims to explore key genes and microRNAs (miRNAs) associated with the pathogenesis of pancreatic cancer. public databases were searched, and the integrated data from The Cancer Genome Atlas and Gene Expression Omnibus database, which leads to the identification of 23 miRNAs that are expressed differently (DE-miRNA). Four of DE-regulated miRNAs (miR-892b-HSA, HSA-miR-194-2, miR-200a-HSA and HSA-miR-194-1), while the DE-19 downregulated miRNA (hsa- Mirza 424, HSA-mir-191, mir-484-HSA, HSA-miR-142, miR-15b-HSA, HSA-miR-450A-1, HSA-mir-423, mir-126-HSA, hsa- Mirza 505, HSA-miR-16-1, HSA-miR-342, miR-130A-HSA, HSA-miR-3613, HSA-miR-450A-2, HSA-miR-26b, miR-451-HSA, HSA- miR-19b-2, HSA-miR-106A and HSA-miR-503) were identified using a cut-off criteria of P <0.05 and | log 2FC |> 1.0. Hsa-miR-3613-5p identified as prognostic DE-miRNA.

Functional enrichment analysis showed that the target gene HSA-miR-3613-5p may be related to p53 signaling pathway. survival analysis performed for the genes in the p53 signaling pathway revealed that the cyclin-dependent kinase 6 and ribonucleoside-diphosphate reductase M2 subunit might be most likely associated with prognostic value. integrated analysis performed in this study shows that the HSA-miR-3613-5p can be used as a potential prognostic marker for pancreatic cancer. Through our research, we have identified general and specific types of miRNA markers and built accurate network-based classifier miRNA, furthering our understanding of the diversity of Nen.

 Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays
Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Phylogenomic Relationships and Evolution of polyploid species Salix RAD Revealed by Sequencing Data

Polyploidy is common in the genus Salix. However, little is known about the origin, ancestry and polyploid genomes of species composition due to lack of suitable molecular markers and analysis tools.

We established a framework phylogenomic including all species of willow bushes described parts of Eurasia. We analyze the composition of the genomes of seven species of willow polyploid compared to the parental diploid species suspected to draw conclusions about their origins and effects of backcrossing and evolution of post-origin.

We applied recently developed programs such as Snapp, Hyde, and SNiPloid to build a bioinformatics pipeline to unravel the complexity of polyploid genomes. RAD sequencing revealed 23 393 320 010 SNP loci and high quality for analysis of the relationship of 35 species Eurasian shrub willow (Salix subg. Chamaetia / Vetrix). willow species seemed dominated polyploid origin allopolyploid.

sequencing unit 33x45 cm

ESEQ1100-SYS ea
EUR 1535.00

sequencing system 20x50 cm

ESEQ1200-SYS ea
EUR 1535.00

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 145.00

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 219.00

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1657.00

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 651.00

SequaGel Sequencing System 1L Kit

NAT1136 1KIT
EUR 143.00

SequaGel Sequencing System 2.2L Kit

NAT1138 EACH
EUR 211.00

PCR Clean Up for DNA Sequencing

BT5100 100preps
EUR 95.68
  • Product category: PCR Related/PCR Kits (Cleanup)

PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
EUR 461.08
  • Product category: PCR Related/PCR Kits (Cleanup)

U1 Primers

MP00001 150 ul / 10 uM
EUR 121.00

SNORD44 Primers

MPH00003 150 ul / 10 uM
EUR 121.00

SNORD47 Primers

MPH00004 150 ul / 10 uM
EUR 121.00

SNORD48 Primers

MPH00005 150 ul / 10 uM
EUR 121.00

RNU43 Primers

MPM00003 150 ul / 10 uM
EUR 121.00

snoRNA142 Primers

MPM00004 150 ul / 10 uM
EUR 121.00

Random Primers

S300 30 µg
EUR 46.00

Random Primers

S305 5x30 µg
EUR 102.00

U6-2 Primers

MPH00001 150 ul / 10 uM
EUR 121.00

U6 snRNA Primers

MPM00002 150 ul / 10 uM
EUR 121.00

U1 snRNA Primers

MPM00006 150 ul / 10 uM
EUR 121.00

mmu-miR-3535 Primers

MPM01245 150 ul / 10 uM
EUR 121.00

mmu-miR-3552 Primers

MPM01250 150 ul / 10 uM
EUR 121.00

mmu-miR-378c Primers

MPM01282 150 ul / 10 uM
EUR 121.00

mmu-miR-378d Primers

MPM01283 150 ul / 10 uM
EUR 121.00

mmu-miR-451a Primers

MPM01313 150 ul / 10 uM
EUR 121.00

mmu-miR-451b Primers

MPM01314 150 ul / 10 uM
EUR 121.00

mmu-miR-466q Primers

MPM01321 150 ul / 10 uM
EUR 121.00

mmu-miR-496b Primers

MPM01360 150 ul / 10 uM
EUR 121.00

mmu-miR-497b Primers

MPM01363 150 ul / 10 uM
EUR 121.00

mmu-miR-5046 Primers

MPM01372 150 ul / 10 uM
EUR 121.00

mmu-miR-5098 Primers

MPM01373 150 ul / 10 uM
EUR 121.00

mmu-miR-5099 Primers

MPM01374 150 ul / 10 uM
EUR 121.00

mmu-miR-5100 Primers

MPM01375 150 ul / 10 uM
EUR 121.00

mmu-miR-5101 Primers

MPM01376 150 ul / 10 uM
EUR 121.00

mmu-miR-5103 Primers

MPM01377 150 ul / 10 uM
EUR 121.00

mmu-miR-5104 Primers

MPM01378 150 ul / 10 uM
EUR 121.00

mmu-miR-5106 Primers

MPM01379 150 ul / 10 uM
EUR 121.00

mmu-miR-5108 Primers

MPM01382 150 ul / 10 uM
EUR 121.00

mmu-miR-5110 Primers

MPM01383 150 ul / 10 uM
EUR 121.00

mmu-miR-5112 Primers

MPM01384 150 ul / 10 uM
EUR 121.00

mmu-miR-5113 Primers

MPM01385 150 ul / 10 uM
EUR 121.00

mmu-miR-5114 Primers

MPM01386 150 ul / 10 uM
EUR 121.00

mmu-miR-5116 Primers

MPM01387 150 ul / 10 uM
EUR 121.00

mmu-miR-5118 Primers

MPM01388 150 ul / 10 uM
EUR 121.00

mmu-miR-5119 Primers

MPM01389 150 ul / 10 uM
EUR 121.00

mmu-miR-5120 Primers

MPM01390 150 ul / 10 uM
EUR 121.00

mmu-miR-5121 Primers

MPM01391 150 ul / 10 uM
EUR 121.00

mmu-miR-5122 Primers

MPM01392 150 ul / 10 uM
EUR 121.00

mmu-miR-5123 Primers

MPM01393 150 ul / 10 uM
EUR 121.00

mmu-miR-5124a Primers

MPM01394 150 ul / 10 uM
EUR 121.00

mmu-miR-5124b Primers

MPM01395 150 ul / 10 uM
EUR 121.00

mmu-miR-5125 Primers

MPM01396 150 ul / 10 uM
EUR 121.00

mmu-miR-5126 Primers

MPM01397 150 ul / 10 uM
EUR 121.00

mmu-miR-5127 Primers

MPM01398 150 ul / 10 uM
EUR 121.00

mmu-miR-5128 Primers

MPM01399 150 ul / 10 uM
EUR 121.00

mmu-miR-5130 Primers

MPM01402 150 ul / 10 uM
EUR 121.00

mmu-miR-5131 Primers

MPM01403 150 ul / 10 uM
EUR 121.00

mmu-miR-5133 Primers

MPM01406 150 ul / 10 uM
EUR 121.00

mmu-miR-5134 Primers

MPM01407 150 ul / 10 uM
EUR 121.00

mmu-miR-5135 Primers

MPM01410 150 ul / 10 uM
EUR 121.00

mmu-miR-5136 Primers

MPM01411 150 ul / 10 uM
EUR 121.00

mmu-miR-5710 Primers

MPM01447 150 ul / 10 uM
EUR 121.00

mmu-miR-6236 Primers

MPM01454 150 ul / 10 uM
EUR 121.00

mmu-miR-6237 Primers

MPM01455 150 ul / 10 uM
EUR 121.00

mmu-miR-6238 Primers

MPM01456 150 ul / 10 uM
EUR 121.00

mmu-miR-6239 Primers

MPM01457 150 ul / 10 uM
EUR 121.00

mmu-miR-6240 Primers

MPM01458 150 ul / 10 uM
EUR 121.00

mmu-miR-6241 Primers

MPM01459 150 ul / 10 uM
EUR 121.00

mmu-miR-6243 Primers

MPM01460 150 ul / 10 uM
EUR 121.00

mmu-miR-6244 Primers

MPM01461 150 ul / 10 uM
EUR 121.00

mmu-miR-6335 Primers

MPM01462 150 ul / 10 uM
EUR 121.00

mmu-miR-6336 Primers

MPM01463 150 ul / 10 uM
EUR 121.00

mmu-miR-6337 Primers

MPM01464 150 ul / 10 uM
EUR 121.00

mmu-miR-6338 Primers

MPM01465 150 ul / 10 uM
EUR 121.00

mmu-miR-6339 Primers

MPM01466 150 ul / 10 uM
EUR 121.00

mmu-miR-6340 Primers

MPM01467 150 ul / 10 uM
EUR 121.00

mmu-miR-6341 Primers

MPM01468 150 ul / 10 uM
EUR 121.00

mmu-miR-6342 Primers

MPM01469 150 ul / 10 uM
EUR 121.00

mmu-miR-6343 Primers

MPM01470 150 ul / 10 uM
EUR 121.00

mmu-miR-6344 Primers

MPM01471 150 ul / 10 uM
EUR 121.00

mmu-miR-6345 Primers

MPM01472 150 ul / 10 uM
EUR 121.00

mmu-miR-6346 Primers

MPM01473 150 ul / 10 uM
EUR 121.00

mmu-miR-6347 Primers

MPM01474 150 ul / 10 uM
EUR 121.00

mmu-miR-6348 Primers

MPM01475 150 ul / 10 uM
EUR 121.00

mmu-miR-6349 Primers

MPM01476 150 ul / 10 uM
EUR 121.00

mmu-miR-6350 Primers

MPM01477 150 ul / 10 uM
EUR 121.00

mmu-miR-6351 Primers

MPM01478 150 ul / 10 uM
EUR 121.00

mmu-miR-6352 Primers

MPM01479 150 ul / 10 uM
EUR 121.00

mmu-miR-6353 Primers

MPM01480 150 ul / 10 uM
EUR 121.00

mmu-miR-6354 Primers

MPM01481 150 ul / 10 uM
EUR 121.00

mmu-miR-6355 Primers

MPM01482 150 ul / 10 uM
EUR 121.00

mmu-miR-6356 Primers

MPM01483 150 ul / 10 uM
EUR 121.00

mmu-miR-6357 Primers

MPM01484 150 ul / 10 uM
EUR 121.00

mmu-miR-6358 Primers

MPM01485 150 ul / 10 uM
EUR 121.00

mmu-miR-6359 Primers

MPM01486 150 ul / 10 uM
EUR 121.00

mmu-miR-6360 Primers

MPM01487 150 ul / 10 uM
EUR 121.00

mmu-miR-6361 Primers

MPM01488 150 ul / 10 uM
EUR 121.00

mmu-miR-6362 Primers

MPM01489 150 ul / 10 uM
EUR 121.00

mmu-miR-6363 Primers

MPM01490 150 ul / 10 uM
EUR 121.00

mmu-miR-6364 Primers

MPM01491 150 ul / 10 uM
EUR 121.00

mmu-miR-6365 Primers

MPM01492 150 ul / 10 uM
EUR 121.00

mmu-miR-6366 Primers

MPM01493 150 ul / 10 uM
EUR 121.00

mmu-miR-6367 Primers

MPM01494 150 ul / 10 uM
EUR 121.00

mmu-miR-6368 Primers

MPM01495 150 ul / 10 uM
EUR 121.00

mmu-miR-6369 Primers

MPM01496 150 ul / 10 uM
EUR 121.00

mmu-miR-6370 Primers

MPM01497 150 ul / 10 uM
EUR 121.00

mmu-miR-6371 Primers

MPM01498 150 ul / 10 uM
EUR 121.00

mmu-miR-6372 Primers

MPM01499 150 ul / 10 uM
EUR 121.00

mmu-miR-6373 Primers

MPM01500 150 ul / 10 uM
EUR 121.00

mmu-miR-6374 Primers

MPM01501 150 ul / 10 uM
EUR 121.00

mmu-miR-6375 Primers

MPM01502 150 ul / 10 uM
EUR 121.00

mmu-miR-6376 Primers

MPM01503 150 ul / 10 uM
EUR 121.00

mmu-miR-6377 Primers

MPM01504 150 ul / 10 uM
EUR 121.00

mmu-miR-6378 Primers

MPM01505 150 ul / 10 uM
EUR 121.00

mmu-miR-6379 Primers

MPM01506 150 ul / 10 uM
EUR 121.00

mmu-miR-6380 Primers

MPM01507 150 ul / 10 uM
EUR 121.00

mmu-miR-6381 Primers

MPM01508 150 ul / 10 uM
EUR 121.00

mmu-miR-6382 Primers

MPM01509 150 ul / 10 uM
EUR 121.00

mmu-miR-6383 Primers

MPM01510 150 ul / 10 uM
EUR 121.00

mmu-miR-6384 Primers

MPM01511 150 ul / 10 uM
EUR 121.00

mmu-miR-6385 Primers

MPM01512 150 ul / 10 uM
EUR 121.00

mmu-miR-6386 Primers

MPM01513 150 ul / 10 uM
EUR 121.00

mmu-miR-6387 Primers

MPM01514 150 ul / 10 uM
EUR 121.00

mmu-miR-6388 Primers

MPM01515 150 ul / 10 uM
EUR 121.00

mmu-miR-6389 Primers

MPM01516 150 ul / 10 uM
EUR 121.00

mmu-miR-6390 Primers

MPM01517 150 ul / 10 uM
EUR 121.00

mmu-miR-6391 Primers

MPM01518 150 ul / 10 uM
EUR 121.00

mmu-miR-6393 Primers

MPM01521 150 ul / 10 uM
EUR 121.00

mmu-miR-6394 Primers

MPM01522 150 ul / 10 uM
EUR 121.00

mmu-miR-6395 Primers

MPM01523 150 ul / 10 uM
EUR 121.00

mmu-miR-6396 Primers

MPM01524 150 ul / 10 uM
EUR 121.00

mmu-miR-6397 Primers

MPM01525 150 ul / 10 uM
EUR 121.00

mmu-miR-6398 Primers

MPM01526 150 ul / 10 uM
EUR 121.00

mmu-miR-6399 Primers

MPM01527 150 ul / 10 uM
EUR 121.00

mmu-miR-6400 Primers

MPM01528 150 ul / 10 uM
EUR 121.00

mmu-miR-6401 Primers

MPM01529 150 ul / 10 uM
EUR 121.00

mmu-miR-6402 Primers

MPM01530 150 ul / 10 uM
EUR 121.00

mmu-miR-6403 Primers

MPM01531 150 ul / 10 uM
EUR 121.00

mmu-miR-6404 Primers

MPM01532 150 ul / 10 uM
EUR 121.00

mmu-miR-6405 Primers

MPM01533 150 ul / 10 uM
EUR 121.00

mmu-miR-6406 Primers

MPM01534 150 ul / 10 uM
EUR 121.00

mmu-miR-6407 Primers

MPM01535 150 ul / 10 uM
EUR 121.00

mmu-miR-6408 Primers

MPM01536 150 ul / 10 uM
EUR 121.00

mmu-miR-6409 Primers

MPM01537 150 ul / 10 uM
EUR 121.00

mmu-miR-6410 Primers

MPM01538 150 ul / 10 uM
EUR 121.00

mmu-miR-6411 Primers

MPM01539 150 ul / 10 uM
EUR 121.00

mmu-miR-6412 Primers

MPM01540 150 ul / 10 uM
EUR 121.00

mmu-miR-6413 Primers

MPM01541 150 ul / 10 uM
EUR 121.00

mmu-miR-6414 Primers

MPM01542 150 ul / 10 uM
EUR 121.00

mmu-miR-6415 Primers

MPM01543 150 ul / 10 uM
EUR 121.00

mmu-miR-6417 Primers

MPM01546 150 ul / 10 uM
EUR 121.00

mmu-miR-6419 Primers

MPM01549 150 ul / 10 uM
EUR 121.00

mmu-miR-6420 Primers

MPM01550 150 ul / 10 uM
EUR 121.00

mmu-miR-6481 Primers

MPM01551 150 ul / 10 uM
EUR 121.00

mmu-miR-6538 Primers

MPM01560 150 ul / 10 uM
EUR 121.00

mmu-miR-6539 Primers

MPM01561 150 ul / 10 uM
EUR 121.00

mmu-miR-6541 Primers

MPM01564 150 ul / 10 uM
EUR 121.00

mmu-miR-7578 Primers

MPM02103 150 ul / 10 uM
EUR 121.00

mmu-miR-8090 Primers

MPM02201 150 ul / 10 uM
EUR 121.00

Allopolyploidization more ancient events were observed during the two hexaploid and one species octoploid, whereas our data suggest more recent origin for tetraploids allopolyploid including parental taxa and identifying suspected. SNiPloid analysis parse different genomic signatures generated from hybrid origin, backcrossing, and the evolution of post-secondary origin in polyploid species. Our RAD sequencing data indicate that willow genome evolution shaped by ancient and recently reticulate, polyploidization, and post-native species differences.