Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Pancreatic cancer is a malignant tumor of the digestive tract, rendering it difficult to make an accurate diagnosis. 5-year survival rate for pancreatic cancer is <1%, and surgical resection is rarely proven to be effective. Therefore, the identification of more effective methods for early detection of pancreatic cancer is an urgent need.

This study aims to explore key genes and microRNAs (miRNAs) associated with the pathogenesis of pancreatic cancer. public databases were searched, and the integrated data from The Cancer Genome Atlas and Gene Expression Omnibus database, which leads to the identification of 23 miRNAs that are expressed differently (DE-miRNA). Four of DE-regulated miRNAs (miR-892b-HSA, HSA-miR-194-2, miR-200a-HSA and HSA-miR-194-1), while the DE-19 downregulated miRNA (hsa- Mirza 424, HSA-mir-191, mir-484-HSA, HSA-miR-142, miR-15b-HSA, HSA-miR-450A-1, HSA-mir-423, mir-126-HSA, hsa- Mirza 505, HSA-miR-16-1, HSA-miR-342, miR-130A-HSA, HSA-miR-3613, HSA-miR-450A-2, HSA-miR-26b, miR-451-HSA, HSA- miR-19b-2, HSA-miR-106A and HSA-miR-503) were identified using a cut-off criteria of P <0.05 and | log 2FC |> 1.0. Hsa-miR-3613-5p identified as prognostic DE-miRNA.

Functional enrichment analysis showed that the target gene HSA-miR-3613-5p may be related to p53 signaling pathway. survival analysis performed for the genes in the p53 signaling pathway revealed that the cyclin-dependent kinase 6 and ribonucleoside-diphosphate reductase M2 subunit might be most likely associated with prognostic value. integrated analysis performed in this study shows that the HSA-miR-3613-5p can be used as a potential prognostic marker for pancreatic cancer. Through our research, we have identified general and specific types of miRNA markers and built accurate network-based classifier miRNA, furthering our understanding of the diversity of Nen.

 Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays
Screening potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarrays

Phylogenomic Relationships and Evolution of polyploid species Salix RAD Revealed by Sequencing Data

Polyploidy is common in the genus Salix. However, little is known about the origin, ancestry and polyploid genomes of species composition due to lack of suitable molecular markers and analysis tools.

We established a framework phylogenomic including all species of willow bushes described parts of Eurasia. We analyze the composition of the genomes of seven species of willow polyploid compared to the parental diploid species suspected to draw conclusions about their origins and effects of backcrossing and evolution of post-origin.

We applied recently developed programs such as Snapp, Hyde, and SNiPloid to build a bioinformatics pipeline to unravel the complexity of polyploid genomes. RAD sequencing revealed 23 393 320 010 SNP loci and high quality for analysis of the relationship of 35 species Eurasian shrub willow (Salix subg. Chamaetia / Vetrix). willow species seemed dominated polyploid origin allopolyploid.

sequencing unit 33x45 cm

ESEQ1100-SYS ea
EUR 1535.00

sequencing system 20x50 cm

ESEQ1200-SYS ea
EUR 1535.00

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 145.00

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 219.00

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1657.00

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 651.00

SequaGel Sequencing System 1L Kit

NAT1136 1KIT
EUR 143.00

SequaGel Sequencing System 2.2L Kit

NAT1138 EACH
EUR 211.00

PCR Clean Up for DNA Sequencing

BT5100 100preps
EUR 95.68
  • Product category: PCR Related/PCR Kits (Cleanup)

PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
EUR 461.08
  • Product category: PCR Related/PCR Kits (Cleanup)

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 480.00

RNU43 Primers

MPM00003 150 ul / 10 uM
EUR 121.00

snoRNA142 Primers

MPM00004 150 ul / 10 uM
EUR 121.00

U1 Primers

MP00001 150 ul / 10 uM
EUR 121.00

SNORD44 Primers

MPH00003 150 ul / 10 uM
EUR 121.00

SNORD47 Primers

MPH00004 150 ul / 10 uM
EUR 121.00

SNORD48 Primers

MPH00005 150 ul / 10 uM
EUR 121.00

Random Primers

S300 30 µg
EUR 46.00

Random Primers

S305 5x30 µg
EUR 102.00

U6 snRNA Primers

MPM00002 150 ul / 10 uM
EUR 121.00

U1 snRNA Primers

MPM00006 150 ul / 10 uM
EUR 121.00

U6-2 Primers

MPH00001 150 ul / 10 uM
EUR 121.00

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 10/pk
EUR 533.00
Description: Sealing Products; Sealing mats - Axygen

mmu-miR-1931 Primers

MPM00151 150 ul / 10 uM
EUR 121.00

mmu-miR-1932 Primers

MPM00152 150 ul / 10 uM
EUR 121.00

mmu-miR-1934 Primers

MPM00155 150 ul / 10 uM
EUR 121.00

mmu-miR-1935 Primers

MPM00156 150 ul / 10 uM
EUR 121.00

mmu-miR-1936 Primers

MPM00157 150 ul / 10 uM
EUR 121.00

mmu-miR-1937a Primers

MPM00158 150 ul / 10 uM
EUR 121.00

mmu-miR-1937b Primers

MPM00159 150 ul / 10 uM
EUR 121.00

mmu-miR-1937c Primers

MPM00160 150 ul / 10 uM
EUR 121.00

mmu-miR-1938 Primers

MPM00161 150 ul / 10 uM
EUR 121.00

mmu-miR-1939 Primers

MPM00162 150 ul / 10 uM
EUR 121.00

mmu-miR-193b Primers

MPM00163 150 ul / 10 uM
EUR 121.00

mmu-miR-194 Primers

MPM00164 150 ul / 10 uM
EUR 121.00

mmu-miR-1940 Primers

MPM00165 150 ul / 10 uM
EUR 121.00

mmu-miR-1942 Primers

MPM00168 150 ul / 10 uM
EUR 121.00

mmu-miR-1943 Primers

MPM00169 150 ul / 10 uM
EUR 121.00

mmu-miR-1944 Primers

MPM00170 150 ul / 10 uM
EUR 121.00

mmu-miR-1945 Primers

MPM00171 150 ul / 10 uM
EUR 121.00

mmu-miR-1946a Primers

MPM00172 150 ul / 10 uM
EUR 121.00

mmu-miR-1946b Primers

MPM00173 150 ul / 10 uM
EUR 121.00

mmu-miR-1947 Primers

MPM00174 150 ul / 10 uM
EUR 121.00

mmu-miR-1948 Primers

MPM00175 150 ul / 10 uM
EUR 121.00

mmu-miR-1949 Primers

MPM00176 150 ul / 10 uM
EUR 121.00

mmu-miR-195 Primers

MPM00177 150 ul / 10 uM
EUR 121.00

mmu-miR-1950 Primers

MPM00178 150 ul / 10 uM
EUR 121.00

mmu-miR-1951 Primers

MPM00179 150 ul / 10 uM
EUR 121.00

mmu-miR-1952 Primers

MPM00180 150 ul / 10 uM
EUR 121.00

mmu-miR-1953 Primers

MPM00181 150 ul / 10 uM
EUR 121.00

mmu-miR-1954 Primers

MPM00182 150 ul / 10 uM
EUR 121.00

mmu-miR-1956 Primers

MPM00185 150 ul / 10 uM
EUR 121.00

mmu-miR-1957 Primers

MPM00186 150 ul / 10 uM
EUR 121.00

mmu-miR-1958 Primers

MPM00187 150 ul / 10 uM
EUR 121.00

mmu-miR-1959 Primers

MPM00188 150 ul / 10 uM
EUR 121.00

mmu-miR-1960 Primers

MPM00189 150 ul / 10 uM
EUR 121.00

mmu-miR-1961 Primers

MPM00190 150 ul / 10 uM
EUR 121.00

mmu-miR-1962 Primers

MPM00191 150 ul / 10 uM
EUR 121.00

mmu-miR-1963 Primers

MPM00192 150 ul / 10 uM
EUR 121.00

mmu-miR-1965 Primers

MPM00195 150 ul / 10 uM
EUR 121.00

mmu-miR-1966 Primers

MPM00196 150 ul / 10 uM
EUR 121.00

mmu-miR-1967 Primers

MPM00197 150 ul / 10 uM
EUR 121.00

mmu-miR-1968 Primers

MPM00198 150 ul / 10 uM
EUR 121.00

mmu-miR-1969 Primers

MPM00199 150 ul / 10 uM
EUR 121.00

mmu-miR-196a Primers

MPM00200 150 ul / 10 uM
EUR 121.00

mmu-miR-196b Primers

MPM00201 150 ul / 10 uM
EUR 121.00

mmu-miR-197 Primers

MPM00202 150 ul / 10 uM
EUR 121.00

mmu-miR-1970 Primers

MPM00203 150 ul / 10 uM
EUR 121.00

mmu-miR-1971 Primers

MPM00204 150 ul / 10 uM
EUR 121.00

mmu-miR-1981 Primers

MPM00205 150 ul / 10 uM
EUR 121.00

mmu-miR-1982.1 Primers

MPM00206 150 ul / 10 uM
EUR 121.00

mmu-miR-1982.2 Primers

MPM00207 150 ul / 10 uM
EUR 121.00

mmu-miR-1983 Primers

MPM00208 150 ul / 10 uM
EUR 121.00

mmu-miR-199b Primers

MPM00211 150 ul / 10 uM
EUR 121.00

mmu-miR-19a Primers

MPM00212 150 ul / 10 uM
EUR 121.00

mmu-miR-19b Primers

MPM00213 150 ul / 10 uM
EUR 121.00

mmu-miR-200a Primers

MPM00214 150 ul / 10 uM
EUR 121.00

mmu-miR-200b Primers

MPM00215 150 ul / 10 uM
EUR 121.00

mmu-miR-200c Primers

MPM00216 150 ul / 10 uM
EUR 121.00

mmu-miR-201 Primers

MPM00217 150 ul / 10 uM
EUR 121.00

mmu-miR-203 Primers

MPM00220 150 ul / 10 uM
EUR 121.00

mmu-miR-204 Primers

MPM00221 150 ul / 10 uM
EUR 121.00

mmu-miR-205 Primers

MPM00222 150 ul / 10 uM
EUR 121.00

mmu-miR-206 Primers

MPM00223 150 ul / 10 uM
EUR 121.00

mmu-miR-207 Primers

MPM00224 150 ul / 10 uM
EUR 121.00

mmu-miR-208b Primers

MPM00227 150 ul / 10 uM
EUR 121.00

mmu-miR-20a Primers

MPM00228 150 ul / 10 uM
EUR 121.00

mmu-miR-20b Primers

MPM00229 150 ul / 10 uM
EUR 121.00

mmu-miR-21 Primers

MPM00230 150 ul / 10 uM
EUR 121.00

mmu-miR-210 Primers

MPM00231 150 ul / 10 uM
EUR 121.00

mmu-miR-211 Primers

MPM00232 150 ul / 10 uM
EUR 121.00

mmu-miR-2136 Primers

MPM00235 150 ul / 10 uM
EUR 121.00

mmu-miR-2137 Primers

MPM00236 150 ul / 10 uM
EUR 121.00

mmu-miR-2139 Primers

MPM00237 150 ul / 10 uM
EUR 121.00

mmu-miR-214 Primers

MPM00238 150 ul / 10 uM
EUR 121.00

mmu-miR-2142 Primers

MPM00239 150 ul / 10 uM
EUR 121.00

mmu-miR-2143 Primers

MPM00240 150 ul / 10 uM
EUR 121.00

mmu-miR-2144 Primers

MPM00241 150 ul / 10 uM
EUR 121.00

mmu-miR-2145 Primers

MPM00242 150 ul / 10 uM
EUR 121.00

mmu-miR-215 Primers

MPM00243 150 ul / 10 uM
EUR 121.00

mmu-miR-216a Primers

MPM00244 150 ul / 10 uM
EUR 121.00

mmu-miR-216b Primers

MPM00245 150 ul / 10 uM
EUR 121.00

mmu-miR-217 Primers

MPM00246 150 ul / 10 uM
EUR 121.00

mmu-miR-218 Primers

MPM00247 150 ul / 10 uM
EUR 121.00

mmu-miR-2182 Primers

MPM00248 150 ul / 10 uM
EUR 121.00

mmu-miR-2183 Primers

MPM00249 150 ul / 10 uM
EUR 121.00

mmu-miR-22 Primers

MPM00252 150 ul / 10 uM
EUR 121.00

mmu-miR-221 Primers

MPM00253 150 ul / 10 uM
EUR 121.00

mmu-miR-222 Primers

MPM00254 150 ul / 10 uM
EUR 121.00

mmu-miR-223 Primers

MPM00255 150 ul / 10 uM
EUR 121.00

mmu-miR-224 Primers

MPM00256 150 ul / 10 uM
EUR 121.00

mmu-miR-23a Primers

MPM00257 150 ul / 10 uM
EUR 121.00

mmu-miR-23b Primers

MPM00258 150 ul / 10 uM
EUR 121.00

mmu-miR-24 Primers

MPM00259 150 ul / 10 uM
EUR 121.00

mmu-miR-25 Primers

MPM00260 150 ul / 10 uM
EUR 121.00

mmu-miR-26a Primers

MPM00261 150 ul / 10 uM
EUR 121.00

mmu-miR-26b Primers

MPM00262 150 ul / 10 uM
EUR 121.00

mmu-miR-27a Primers

MPM00263 150 ul / 10 uM
EUR 121.00

mmu-miR-27b Primers

MPM00264 150 ul / 10 uM
EUR 121.00

mmu-miR-28 Primers

MPM00265 150 ul / 10 uM
EUR 121.00

mmu-miR-2861 Primers

MPM00266 150 ul / 10 uM
EUR 121.00

mmu-miR-293 Primers

MPM00275 150 ul / 10 uM
EUR 121.00

mmu-miR-294 Primers

MPM00276 150 ul / 10 uM
EUR 121.00

mmu-miR-295 Primers

MPM00277 150 ul / 10 uM
EUR 121.00

mmu-miR-297a Primers

MPM00280 150 ul / 10 uM
EUR 121.00

mmu-miR-297c Primers

MPM00283 150 ul / 10 uM
EUR 121.00

mmu-miR-298 Primers

MPM00284 150 ul / 10 uM
EUR 121.00

mmu-miR-299 Primers

MPM00285 150 ul / 10 uM
EUR 121.00

mmu-miR-29a Primers

MPM00286 150 ul / 10 uM
EUR 121.00

mmu-miR-29b Primers

MPM00287 150 ul / 10 uM
EUR 121.00

mmu-miR-29c Primers

MPM00288 150 ul / 10 uM
EUR 121.00

mmu-miR-300 Primers

MPM00289 150 ul / 10 uM
EUR 121.00

mmu-miR-301a Primers

MPM00290 150 ul / 10 uM
EUR 121.00

mmu-miR-301b Primers

MPM00291 150 ul / 10 uM
EUR 121.00

mmu-miR-302a Primers

MPM00292 150 ul / 10 uM
EUR 121.00

mmu-miR-302b Primers

MPM00293 150 ul / 10 uM
EUR 121.00

mmu-miR-302c Primers

MPM00294 150 ul / 10 uM
EUR 121.00

mmu-miR-302d Primers

MPM00295 150 ul / 10 uM
EUR 121.00

mmu-miR-3058 Primers

MPM00298 150 ul / 10 uM
EUR 121.00

mmu-miR-3059 Primers

MPM00299 150 ul / 10 uM
EUR 121.00

mmu-miR-3060 Primers

MPM00300 150 ul / 10 uM
EUR 121.00

mmu-miR-3062 Primers

MPM00303 150 ul / 10 uM
EUR 121.00

mmu-miR-3063 Primers

MPM00304 150 ul / 10 uM
EUR 121.00

mmu-miR-3065 Primers

MPM00307 150 ul / 10 uM
EUR 121.00

mmu-miR-3066 Primers

MPM00308 150 ul / 10 uM
EUR 121.00

mmu-miR-3067 Primers

MPM00309 150 ul / 10 uM
EUR 121.00

mmu-miR-3068 Primers

MPM00310 150 ul / 10 uM
EUR 121.00

mmu-miR-3070a Primers

MPM00313 150 ul / 10 uM
EUR 121.00

mmu-miR-3071 Primers

MPM00316 150 ul / 10 uM
EUR 121.00

mmu-miR-3075 Primers

MPM00322 150 ul / 10 uM
EUR 121.00

mmu-miR-3077 Primers

MPM00325 150 ul / 10 uM
EUR 121.00

mmu-miR-3078 Primers

MPM00326 150 ul / 10 uM
EUR 121.00

mmu-miR-3081 Primers

MPM00331 150 ul / 10 uM
EUR 121.00

mmu-miR-3083 Primers

MPM00334 150 ul / 10 uM
EUR 121.00

mmu-miR-3084 Primers

MPM00335 150 ul / 10 uM
EUR 121.00

mmu-miR-3087 Primers

MPM00340 150 ul / 10 uM
EUR 121.00

mmu-miR-3088 Primers

MPM00341 150 ul / 10 uM
EUR 121.00

mmu-miR-3090 Primers

MPM00344 150 ul / 10 uM
EUR 121.00

mmu-miR-3092 Primers

MPM00347 150 ul / 10 uM
EUR 121.00

mmu-miR-3094 Primers

MPM00350 150 ul / 10 uM
EUR 121.00

Allopolyploidization more ancient events were observed during the two hexaploid and one species octoploid, whereas our data suggest more recent origin for tetraploids allopolyploid including parental taxa and identifying suspected. SNiPloid analysis parse different genomic signatures generated from hybrid origin, backcrossing, and the evolution of post-secondary origin in polyploid species. Our RAD sequencing data indicate that willow genome evolution shaped by ancient and recently reticulate, polyploidization, and post-native species differences.