Motivation: The tumor is composed of different cancer cell population (clones), which continues to adapt to their local microenvironment. the standard method for deconvolution clonal mutation group seeks to identify and estimate the prevalence of each group in the tumor, while taking into account the purity and copy number profile. This method has been applied to a cross-sectional and longitudinal data after removing information about the time of sample collection.
Two key question is how we can combine the information in our analyzes and there are benefits in doing so? Results: We developed clonal deconvolution method, which combines explicit temporal distance longitudinal tumor samples. By combining the Dirichlet Process Mixture Model Gaussian Process priors and used as an input sequence is rarely collected several samples, our method can reconstruct the temporal profile mutation abundance of each cluster is supported by data as a continuous function of time.
We refer to our method on the whole genome, whole exome and sequencing data targeted from patients with chronic lymphocytic leukemia, the data biopsy fluid of patients with melanoma and synthetic data and we found that combining information about a collection of networks improve the performance of the model, as long as the data volume and complexity is available for the free estimate model parameters. Thus, our approach is very useful when collecting a relatively long sequences of tumor samples feasible, such as in liquid cancers (eg, leukemia) and liquid biopsy.
Bactrian camel population size smaller than the dromedary, and they are distributed in the cold and mountain regions and also at risk of extinction in some countries such as Iran. To identify and investigate genome-wide variation, whole-genome sequencing of two Bactrian camels Iran do with the scope 37.4- and 42.6-fold for the first time. Along with Iran Bactrian camel, sequencing data from two domestic and two Mongolian wild Bactrian camel is stored in the NCBI reanalyzed.
A statistical approach for tracking clonal dynamics in cancer using longitudinal next-generation sequencing data
scPADGRN: An asphalt ADMM approach to reconstruct gene regulatory networks dynamically using RNA sequencing data-single cell
development and cell differentiation disease both involve dynamic changes; Therefore, reconstruction of dynamic gene regulatory network (DGRNs) is an important issue but it is difficult in biological systems. With recent advances in single-cell technical RNA sequencing (scRNA-seq), a large volume of data scRNA-seq is being obtained for the various processes. However, current methods of concluding DGRNs of the bulk sample may not be suitable for data-seq scRNA.
In this work, we present scPADGRN, inference DGRN new method using “time-series” scRNA-seq data. scPADGRN combining the method of alternating directions asphalt multiplier by grouping cells for reconstruction DGRN. It shows the advantages in accuracy, robustness and fast convergence. In addition, so-called quantitative index Differentiation Gene Interactions Enrichment (DGIE) served to measure the interaction enrichment of genes related to differentiation.
From the score DGIE of the relevant subnetwork, we conclude that the function of embryonic stem (ES) cells are most active initially and may gradually fade over time. Communication power of genes contribute to note that facilitate the differentiation of cells increased from ES cells differentiated into severe cells.
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
MT1L (Metallothionein 1L (Gene/Pseudogene)) is a Protein Coding gene. Metallothioneins have a high content of cysteine residues that bind various heavy metals
these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
MT1L (Metallothionein 1L (Gene/Pseudogene)) is a Protein Coding gene. Metallothioneins have a high content of cysteine residues that bind various heavy metals
these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
MT1L (Metallothionein 1L (Gene/Pseudogene)) is a Protein Coding gene. Metallothioneins have a high content of cysteine residues that bind various heavy metals
these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Rabbit Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Retinoic Acid Early Transcript 1L ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Retinoic Acid Early Transcript 1L ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Retinoic Acid Early Transcript 1L ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Retinoic Acid Early Transcript 1L ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Retinoic Acid Early Transcript 1L ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
Description: Lab Equipment; Constant Temperature Equipment
We also identified some of the genes responsible for the change in the score DGIE occur during cell differentiation is based on three single-cell dataset real. Our results show that single-cell analysis is based on inference network coupled with quantitative calculations can reveal key transcriptional regulators involved in cell differentiation and disease progression.
MarK
1L SequaGel XR 
1L SequaGel UreaGel Concentrate 
1L SequaGel UreaGel-6 
1L SequaGel UreaGel-8 
1L SequaGel UreaGel Diluent 
sequencing system 20x50 cm 
UreaGel 29:1 System 1L Kit 
REAGENT BOTTLE, 1L, PP 
BEAKER,1L,PLASTIC,LOW FORM,PP,6/6 
BEAKER,1L,PLASTIC,LOW FORM,PFA,1/6 
BEAKER,1L,WITH HANDLE AND SPOUT,PP,12/12 
SequaGel MD SSCP Kit 
SequaGel MD Heteroduplex Kit 
CYLINDER,GRAD,1L,PLASTIC,SINGLE METRIC SCALE,PP,TC W/FUNNEL,1/6 
100ML SequaGel Buffer 
450ML SequaGel XR 
VOL FLASK,1L,PLASTIC,CLASS A,PMP,24/29 TAPERED PP STOPPER,1/3 
VOL FLASK,1L,PLASTIC,CLASS B,PP,24/29 TAPERED PP STOPPER,1/3 )
PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) 
Chymotrypsin for Sequencing grade 
sequencing unit 33x45 cm 
Trypin for Mass & Sequencing )
PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) 
450ML SequaGel UreaGel Concentrate 
200ML SequaGel UreaGel Buffer 
450ML SequaGel UreaGel-6 
450ML SequaGel UreaGel-8 
450ML SequaGel UreaGel Diluent 
90ML SequaGel Complete Buffer 
100ML SequaGel XR Concentrate 
450ML SequaGel XR Concentrate 
1L HarrisHematoxylin 
1L Autofluor )
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) )
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) 
DNA Library Prep Kit for IIlumina Sequencing 
200ML SequaGel UreaGel Complete Buffer 
200ML SequaGel MD Monomer Solution 
1L Opticlear Sample Kit I 
1L Opticlear Sample Kit II 
PLASTIC CLAMP, 1L 
SLINGSHOT-1L Antibody )
Dermcidin-1L (human) 
1L-chiro-Inositol 
1L Tween 20 
1L ProtoBlue Safe 
1L ProtoStain Blue 
1L AcrylaGel 30% 
1L TBE 10X 
1L TBE 5X 
1L Denaturation Solution 
1L Neutralization Solution )
1L ProtoGel (30%) )
1L ProtoGel (40%) 
1L Scotts Tapwater 
1L Oxosol 306 
Beaker Brushes, 1L 
PCR Clean Up for DNA Sequencing 
1.2ML SequaGel MD SSCP Stop Solution 
Frit Kit 
Column Packing Kit 
Human Metallothionein- 1L, MT1L ELISA KIT ELISA Kit)
Human Metallothionein 1L(MT1L)ELISA Kit 
PCR Mycoplasma Detection Kit 
CELL EXPANSION BAG, 1L 
SLINGSHOT-1L Conjugated Antibody )
InstantBlue 1L (Instant Blue) 
1L DEPC Treated Water 
1L Bis-AcrylaGel 2% 
1L TAE Buffer 50X 
1L SSC Buffer 20X 
1L ProtoGel Resolving Buffer 
1L EDTA 0.5M Solution 
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit 
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit 
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit 
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit 
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit 
Human Protein phosphatase 1L, PPM1L ELISA KIT 
Bovine Protein phosphatase 1L, PPM1L ELISA KIT 
Mouse Protein phosphatase 1L, Ppm1l ELISA KIT )
ELISA kit for Human Metallothionein-1L (MT1L) 
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector 
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector 
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector )
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) -T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit -EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit -T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit -EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit 
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector 
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector 
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector  and Fast ligase (2.5ul)])
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] 
CLAMP FOR 1L ERLENMYER FLASK 
ICE PAN, MINI, 1L GREEN 
ICE PAN, MINI, 1L BLUE 
ICE PAN, MINI, 1L RED 
ICE PAN, MINI, 1L ORANGE 
ICE PAN, MINI, 1L PINK 
ICE PAN, MINI, 1L PURPLE 
COLLECTION CONTAINER 1L, EVA FILM 
Recombinant human Protein phosphatase 1L 
1L UreaGel 29:1 Concentrate )
1L AccuGel 19:1 (30%) )
1L AccuGel 19:1 (40%) )
1L AccuGel 29:1 (30%) )
1L AccuGel 29:1 (40%) 
1L Sodium Chloride 0.009 Solution 
1L Sodium Chloride 1M Solution 
1L Potassium Chloride 1M Solution 
ProtoBlock System )
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) )
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) 
Rabbit Retinoic Acid Early Transcript 1L ELISA kit 
Rat Retinoic Acid Early Transcript 1L ELISA kit 
Mouse Retinoic Acid Early Transcript 1L ELISA kit 
Human Retinoic Acid Early Transcript 1L ELISA kit 
Goat Retinoic Acid Early Transcript 1L ELISA kit 
Dog Retinoic Acid Early Transcript 1L ELISA kit 
Monkey Retinoic Acid Early Transcript 1L ELISA kit 
Pig Retinoic Acid Early Transcript 1L ELISA kit , Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) , Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) , Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) 
BCA Protein Assay Kit 
PLATFORM WITH 6X 1L FLASK CLAMPS 