Motivation: The tumor is composed of different cancer cell population (clones), which continues to adapt to their local microenvironment. the standard method for deconvolution clonal mutation group seeks to identify and estimate the prevalence of each group in the tumor, while taking into account the purity and copy number profile. This method has been applied to a cross-sectional and longitudinal data after removing information about the time of sample collection.

Two key question is how we can combine the information in our analyzes and there are benefits in doing so?
Results: We developed clonal deconvolution method, which combines explicit temporal distance longitudinal tumor samples. By combining the Dirichlet Process Mixture Model Gaussian Process priors and used as an input sequence is rarely collected several samples, our method can reconstruct the temporal profile mutation abundance of each cluster is supported by data as a continuous function of time.

We refer to our method on the whole genome, whole exome and sequencing data targeted from patients with chronic lymphocytic leukemia, the data biopsy fluid of patients with melanoma and synthetic data and we found that combining information about a collection of networks improve the performance of the model, as long as the data volume and complexity is available for the free estimate model parameters. Thus, our approach is very useful when collecting a relatively long sequences of tumor samples feasible, such as in liquid cancers (eg, leukemia) and liquid biopsy.

Bactrian camel population size smaller than the dromedary, and they are distributed in the cold and mountain regions and also at risk of extinction in some countries such as Iran. To identify and investigate genome-wide variation, whole-genome sequencing of two Bactrian camels Iran do with the scope 37.4- and 42.6-fold for the first time. Along with Iran Bactrian camel, sequencing data from two domestic and two Mongolian wild Bactrian camel is stored in the NCBI reanalyzed.

scPADGRN: An asphalt ADMM approach to reconstruct gene regulatory networks dynamically using RNA sequencing data-single cell

development and cell differentiation disease both involve dynamic changes; Therefore, reconstruction of dynamic gene regulatory network (DGRNs) is an important issue but it is difficult in biological systems. With recent advances in single-cell technical RNA sequencing (scRNA-seq), a large volume of data scRNA-seq is being obtained for the various processes. However, current methods of concluding DGRNs of the bulk sample may not be suitable for data-seq scRNA.

In this work, we present scPADGRN, inference DGRN new method using “time-series” scRNA-seq data. scPADGRN combining the method of alternating directions asphalt multiplier by grouping cells for reconstruction DGRN. It shows the advantages in accuracy, robustness and fast convergence. In addition, so-called quantitative index Differentiation Gene Interactions Enrichment (DGIE) served to measure the interaction enrichment of genes related to differentiation.

From the score DGIE of the relevant subnetwork, we conclude that the function of embryonic stem (ES) cells are most active initially and may gradually fade over time. Communication power of genes contribute to note that facilitate the differentiation of cells increased from ES cells differentiated into severe cells.

1L SequaGel XR
NAT1162	National Diagnostics	1L	EUR 138

1L SequaGel UreaGel Concentrate
NAT1134	National Diagnostics	1L	EUR 134

1L SequaGel UreaGel-6
NAT1146	National Diagnostics	1L	EUR 126

1L SequaGel UreaGel-8
NAT1150	National Diagnostics	1L	EUR 126

1L SequaGel UreaGel Diluent
NAT1154	National Diagnostics	1L	EUR 86

sequencing system 20x50 cm
ESEQ1200-SYS	Consort	ea	EUR 1535

UreaGel 29:1 System 1L Kit
NAT1128	National Diagnostics	EACH	EUR 143

REAGENT BOTTLE, 1L, PP
1500P-1L	CORNING	10/pk	EUR 100
Description: Reusable Plastics; Resusable Bottles

BEAKER,1L,PLASTIC,LOW FORM,PP,6/6
1000P-1L	CORNING	6/pk	EUR 63
Description: Reusable Plastics; Resuable Beakers

BEAKER,1L,PLASTIC,LOW FORM,PFA,1/6
1003P-1L	CORNING	1/pk	EUR 653
Description: Reusable Plastics; Resuable Beakers

BEAKER,1L,WITH HANDLE AND SPOUT,PP,12/12
1015P-1L	CORNING	12/pk	EUR 116
Description: Reusable Plastics; Resuable Beakers

SequaGel MD SSCP Kit
NAT1170	National Diagnostics	1KIT	EUR 211

SequaGel MD Heteroduplex Kit
NAT1172	National Diagnostics	EACH	EUR 221

CYLINDER,GRAD,1L,PLASTIC,SINGLE METRIC SCALE,PP,TC W/FUNNEL,1/6
3022P-1L	CORNING	1/pk	EUR 161
Description: Reusable Plastics; Reusable Cylinders

100ML SequaGel Buffer
NAT1140	National Diagnostics	100ML	EUR 67

450ML SequaGel XR
NAT1160	National Diagnostics	450ML	EUR 105

VOL FLASK,1L,PLASTIC,CLASS A,PMP,24/29 TAPERED PP STOPPER,1/3
5640P-1L	CORNING	1/pk	EUR 133
Description: Reusable Plastics; Reusable Volumetric Flasks

VOL FLASK,1L,PLASTIC,CLASS B,PP,24/29 TAPERED PP STOPPER,1/3
5641P-1L	CORNING	1/pk	EUR 121
Description: Reusable Plastics; Reusable Volumetric Flasks

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN400A-KIT	SBI	1 Kit	EUR 2798
Category: PinPoint Integrase Tools

Chymotrypsin for Sequencing grade
C4001-010	GenDepot	4x25ug	EUR 313

Chymotrypsin for Sequencing grade
C4001-100	GenDepot	100ug	EUR 286

sequencing unit 33x45 cm
ESEQ1100-SYS	Consort	ea	EUR 1535

Trypin for Mass & Sequencing
T9600-025	GenDepot	25ug	EUR 145

Trypin for Mass & Sequencing
T9600-100	GenDepot	100ug	EUR 219

Trypin for Mass & Sequencing
T9600-112	GenDepot	12x100ug	EUR 1657

Trypin for Mass & Sequencing
T9600-400	GenDepot	4x100ug	EUR 651

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN300A-KIT	SBI	1 Kit	EUR 2798
Category: PinPoint Integrase Tools

450ML SequaGel UreaGel Concentrate
NAT1132	National Diagnostics	450ML	EUR 105

200ML SequaGel UreaGel Buffer
NAT1142	National Diagnostics	200ML	EUR 70

450ML SequaGel UreaGel-6
NAT1144	National Diagnostics	450ML	EUR 96

450ML SequaGel UreaGel-8
NAT1148	National Diagnostics	450ML	EUR 96

450ML SequaGel UreaGel Diluent
NAT1152	National Diagnostics	450ML	EUR 75

90ML SequaGel Complete Buffer
NAT1156	National Diagnostics	90ML	EUR 66

100ML SequaGel XR Concentrate
NAT1164	National Diagnostics	100ML	EUR 107

450ML SequaGel XR Concentrate
NAT1166	National Diagnostics	450ML	EUR 301

1L HarrisHematoxylin
NAT1338	National Diagnostics	1L	EUR 123

1L Autofluor
NAT1446	National Diagnostics	1L	EUR 216

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN410A-KIT	SBI	1 Kit	EUR 4335
Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN412A-KIT	SBI	1 Kit	EUR 4335
Category: PinPoint Integrase Tools

DNA Library Prep Kit for IIlumina Sequencing
K1475-12	Biovision	12 Rxns	EUR 480

200ML SequaGel UreaGel Complete Buffer
NAT1158	National Diagnostics	200ML	EUR 71

200ML SequaGel MD Monomer Solution
NAT1168	National Diagnostics	200ML	EUR 184

1L Opticlear Sample Kit I
NAT1494	National Diagnostics	1L	EUR 162

1L Opticlear Sample Kit II
NAT1496	National Diagnostics	1L	EUR 179

PLASTIC CLAMP, 1L
480152	CORNING	1/pk	EUR 73
Description: Lab Equipment; Constant Temperature Equipment

SLINGSHOT-1L Antibody
48407-100ul	SAB	100ul	EUR 333

SLINGSHOT-1L Antibody
48407-50ul	SAB	50ul	EUR 239

SLINGSHOT-1L Antibody
abx016133-100ug	Abbexa	100 ug	EUR 411
Shipped within 5-10 working days.

SLINGSHOT-1L Antibody
abx224128-100ug	Abbexa	100 ug	EUR 411
Shipped within 5-10 working days.

Dermcidin-1L (human)
H-7316.0500	Bachem	0.5mg	EUR 177
Description: Sum Formula: C210H359N57O71; CAS# [478898-18-9] net

Dermcidin-1L (human)
H-7316.1000	Bachem	1.0mg	EUR 294
Description: Sum Formula: C210H359N57O71; CAS# [478898-18-9] net

1L-chiro-Inositol
GC7626-100MG	Glentham Life Sciences	100 mg	EUR 102

1L-chiro-Inositol
GC7626-1G	Glentham Life Sciences	1 g	EUR 269

1L Tween 20
NAT1084	National Diagnostics	1L	EUR 141

1L ProtoBlue Safe
NAT1108	National Diagnostics	1L	EUR 133

1L ProtoStain Blue
NAT1112	National Diagnostics	1L	EUR 137

1L AcrylaGel 30%
NAT1118	National Diagnostics	1L	EUR 148

1L TBE 10X
NAT1196	National Diagnostics	1L	EUR 82

1L TBE 5X
NAT1200	National Diagnostics	1L	EUR 77

1L Denaturation Solution
NAT1232	National Diagnostics	1L	EUR 85

1L Neutralization Solution
NAT1234	National Diagnostics	1L	EUR 85

1L ProtoGel (30%)
NAT1260	National Diagnostics	1L	EUR 156

1L ProtoGel (40%)
NAT1264	National Diagnostics	1L	EUR 170

1L Scotts Tapwater
NAT1342	National Diagnostics	1L	EUR 75

1L Oxosol 306
NAT1382	National Diagnostics	1L	EUR 112

Beaker Brushes, 1L
BR007-1K	Bio Basic	1 UNIT	EUR 53.48
Product category: Labware/Lab Accessories/Brushes

PCR Clean Up for DNA Sequencing
BT5100	Bio Basic	100preps	EUR 95.68
Product category: PCR Related/PCR Kits (Cleanup)

PCR Clean Up for DNA Sequencing
BT5101	Bio Basic	1000Preps, 1000prep	EUR 461.08
Product category: PCR Related/PCR Kits (Cleanup)

1.2ML SequaGel MD SSCP Stop Solution
NAT1174	National Diagnostics	1.2ML	EUR 71

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit
PACK-KIT	Next Advance	1pack	EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Human Metallothionein- 1L, MT1L ELISA KIT
ELI-36664h	Lifescience Market	96 Tests	EUR 824

Human Metallothionein 1L(MT1L)ELISA Kit
QY-E02761	Qayee Biotechnology	96T	EUR 361

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 266

CELL EXPANSION BAG, 1L
91-200-85	CORNING	1/pk	EUR 112
Description: Flexible Containers; Bioprocess Bags and Parts

SLINGSHOT-1L Conjugated Antibody
C48407	SAB	100ul	EUR 397

InstantBlue 1L (Instant Blue)
ISB1L	Expedeon	1L bottle	EUR 165
Shelf time: 2 years

1L DEPC Treated Water
NAT1104	National Diagnostics	1L	EUR 100

1L Bis-AcrylaGel 2%
NAT1122	National Diagnostics	1L	EUR 93

1L TAE Buffer 50X
NAT1222	National Diagnostics	1L	EUR 124

1L SSC Buffer 20X
NAT1224	National Diagnostics	1L	EUR 82

1L ProtoGel Resolving Buffer
NAT1268	National Diagnostics	1L	EUR 107

1L EDTA 0.5M Solution
NAT1282	National Diagnostics	1L	EUR 153

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT	SBI	1 kit (10 rxn)	EUR 1110
Category: Cas9

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

Human Protein phosphatase 1L, PPM1L ELISA KIT
ELI-45425h	Lifescience Market	96 Tests	EUR 824

Bovine Protein phosphatase 1L, PPM1L ELISA KIT
ELI-36194b	Lifescience Market	96 Tests	EUR 928

Mouse Protein phosphatase 1L, Ppm1l ELISA KIT
ELI-36546m	Lifescience Market	96 Tests	EUR 865

ELISA kit for Human Metallothionein-1L (MT1L)
KTE61511-48T	Abbkine	48T	EUR 332
MT1L (Metallothionein 1L (Gene/Pseudogene)) is a Protein Coding gene. Metallothioneins have a high content of cysteine residues that bind various heavy metals these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Metallothionein-1L (MT1L)
KTE61511-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2115
MT1L (Metallothionein 1L (Gene/Pseudogene)) is a Protein Coding gene. Metallothioneins have a high content of cysteine residues that bind various heavy metals these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Metallothionein-1L (MT1L)
KTE61511-96T	Abbkine	96T	EUR 539
MT1L (Metallothionein 1L (Gene/Pseudogene)) is a Protein Coding gene. Metallothioneins have a high content of cysteine residues that bind various heavy metals these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT	SBI	1 Kit	EUR 805
Category: Cas9

We also identified some of the genes responsible for the change in the score DGIE occur during cell differentiation is based on three single-cell dataset real. Our results show that single-cell analysis is based on inference network coupled with quantitative calculations can reveal key transcriptional regulators involved in cell differentiation and disease progression.