The recent emergence of previously unknown coronavirus (SARS-CoV-2), first identified in the Chinese city of Wuhan in December 2019 has had a major public health and economic consequences. Although 61 888 confirmed cases were reported in Brazil by 28 April 2020, little is known about the SARS-CoV-2 epidemic in this country.

To better understand the recent epidemic in both populous state in southeast Brazil – Minas Gerais (MG) – we sequenced the complete 40 SARS-CoV-2 genome of MG case and reviewing epidemiological data from three Brazilian states. Both the analysis of genomic and geographical distribution of the reported cases provide evidence for multiple independent introductions MG.

epidemiological estimates of the number of reproduction (R) use different data sources and the theoretical assumptions indicate a potential ongoing transmission of the virus even though the reduction R of the first reported cases of the end of April 2020. Estimated date of SARS-CoV-2 introduction to Brazil is consistent with epidemiological data from the first case of travelers returning from Lombardy, Italy. These findings highlight the nature of the epidemic in COVID-19 MG and reinforces the need for real-time and continuously genome surveillance strategies to better understand and prepare for the deployment of emerging pathogenic virus epidemic.

Development of new approaches to regulate the expression of angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2) is becoming increasingly important in the context of the ongoing 19th COVID pandemic because this enzyme plays an important role in cell infection. In this work we are looking for suspected ACE2 expression and regulatory networks TMPRSS2 mediated by various miRNA isoforms (isomiR) throughout the different human organs using the data miRNA / mRNA sequencing paired publicly available from The Cancer Genome Atlas (TCGA) project.

As a result, we identified several families miRNA targeting ACE2 and TMPRSS2 gene in several tissues. Specifically, we found that the lysine specific demethylase 5B (JARID1B), encoded by the gene KDM5B, can indirectly affect the expression of ACE2 / TMPRSS2 by repressing transcription of HSA-let-7e / HSA-miR-125A and HSA-miR-141 / HSA -mir-200 gene targeting miRNA family.

Development and validation of Houston Methodist Variant Viewer version 3: update our application for next-generation sequencing data interpretation

Objective: Informatics tools support generation sequencing workflows are essential to provide timely interpretation of somatic variants in cancer. Here, we explain the significant updates to our development laboratory and bioinformatics pipeline data management application called Houston Methodist Varian Viewer (HMVV).

Materials and methods: We collect feature requests and suggestions for improvement workflows from end users of version 1.5 HMVV 1. Over the years, we iteratively apply these features in five successive updates to HMVV version 3.

Results: We improve application performance and data throughput while reducing the chance for manual data entry errors. We enabled end-user workflows for pipeline monitoring, variants of interpretation and explanation, and integration with our laboratory information system. maintenance enhanced through improved reporting system defects, high data security, and improved code modularity and environmental systems.

EpiNext High-Sensitivity Bisulfite-Seq Kit (Illumina)
P-1056A	EpiGentek	12 Reactions	EUR 1150

EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)
P-9007	EpiGentek	Ask for price Ask for price Ask for price	12 reactions 12 reactions 24 reactions

EpiArt DNA Methylation Bisulfite Kit
EM101-01	Vazyme	50 rxn	EUR 289.2

EpiArt DNA Methylation Bisulfite Kit
EM101-02	Vazyme	200 rxn	EUR 730.8

Sodium Bisulfite
41900224-1	Glycomatrix	25 g	EUR 25.16

Sodium Bisulfite
41900224-2	Glycomatrix	50 g	EUR 36.41

Sodium Bisulfite
41900224-3	Glycomatrix	100 g	EUR 58.75

Sodium bisulfite
abx182647-5g	Abbexa	5 g	EUR 226.8

Sodium bisulfite
GK7777	Glentham Life Sciences	100g	EUR 115.8

Sodium bisulfite
GK7777-1	Glentham Life Sciences	1	EUR 126.5

Sodium bisulfite
GK7777-100	Glentham Life Sciences	100	EUR 23.7

Sodium bisulfite
GK7777-1KG	Glentham Life Sciences	1 kg	EUR 189.6

Sodium bisulfite
GK7777-500	Glentham Life Sciences	500	EUR 71.1

Sodium bisulfite
GK7777-500G	Glentham Life Sciences	500 g	EUR 122.4

Sodium bisulfite
S04360	Pfaltz & Bauer	500G	EUR 207.5

Sodium bisulfite
SB0834	Bio Basic	500g	EUR 79.66

Sodium bisulfite
abx182647-96tests	Abbexa	96 tests	EUR 162.5

Nojirimycin Bisulfite
MBS406003-5mg	MyBiosource	5mg	EUR 180

Nojirimycin Bisulfite
MBS406003-5x5mg	MyBiosource	5x5mg	EUR 750

EpiArt Magnetic DNA Methylation Bisulfite Kit
EM103-00-10rxns	Vazyme	10 rxns	EUR 1.09

EpiArt Magnetic DNA Methylation Bisulfite Kit
EM103-01-50rxns	Vazyme	50 rxns	EUR 73.58

EpiArt Magnetic DNA Methylation Bisulfite Kit
EM103-02-200rxns	Vazyme	200 rxns	EUR 247.43

Glyoxal sodium bisulfite
G04628	Pfaltz & Bauer	100G	EUR 172.6

Sodium Bisulfite Solution
SBC125	ScyTek Laboratories	125 ml	EUR 11.57

Sodium Bisulfite Solution
SBC500	ScyTek Laboratories	500 ml	EUR 27

Sodium Bisulfite Solution
SBC999	ScyTek Laboratories	1000 ml	EUR 51.42

Menadione sodium bisulfite
GA0934	Glentham Life Sciences	25g	EUR 241.46

Menadione bisulfite (sodium)
HY-B1897A	MedChemExpress	10mM/1mL	EUR 135.6

Menadione sodium bisulfite
GA0934-100	Glentham Life Sciences	100	EUR 263.7

Menadione sodium bisulfite
GA0934-25	Glentham Life Sciences	25	EUR 30.1

Menadione sodium bisulfite
M080-25G	TOKU-E	25 g	EUR 29.67
Description: C11H9N/AO5S

Menadione sodium bisulfite
M080-4x25G	TOKU-E	4 x 25 g	EUR 79.24
Description: C11H9N/AO5S

Menadione bisulfite sodium
T5963-10mg	TargetMol Chemicals	10mg	Ask for price
Description: Menadione bisulfite sodium

Menadione bisulfite sodium
T5963-1g	TargetMol Chemicals	1g	Ask for price
Description: Menadione bisulfite sodium

Menadione bisulfite sodium
T5963-1mg	TargetMol Chemicals	1mg	Ask for price
Description: Menadione bisulfite sodium

Menadione bisulfite sodium
T5963-50mg	TargetMol Chemicals	50mg	Ask for price
Description: Menadione bisulfite sodium

Menadione bisulfite sodium
T5963-5mg	TargetMol Chemicals	5mg	Ask for price
Description: Menadione bisulfite sodium

Menadione sodium bisulfite
MBS3610516-100mg	MyBiosource	100mg	EUR 290

Menadione sodium bisulfite
MBS3610516-10mg	MyBiosource	10mg	EUR 200

Menadione sodium bisulfite
MBS3610516-200mg	MyBiosource	200mg	EUR 345

Menadione sodium bisulfite
MBS3610516-25mg	MyBiosource	25mg	EUR 220

Menadione sodium bisulfite
MBS3610516-50mg	MyBiosource	50mg	EUR 245

Menadione sodium bisulfite
MBS5752240-100mg	MyBiosource	100mg	EUR 220

Menadione sodium bisulfite
MBS5752240-10mg	MyBiosource	10mg	EUR 145

Menadione sodium bisulfite
MBS5752240-200mg	MyBiosource	200mg	EUR 280

Menadione sodium bisulfite
MBS5752240-25mg	MyBiosource	25mg	EUR 155

Menadione sodium bisulfite
MBS5752240-50mg	MyBiosource	50mg	EUR 180

BisulFlash DNA Bisulfite Conversion Easy Kit
P-1054	EpiGentek	50 reactions	EUR 303.6

D-Mannojirimycin Bisulfite
M165150	Toronto Research Chemicals	10mg	EUR 184

Methylamp RNA Bisulfite Conversion Kit
P-9003	EpiGentek	Ask for price Ask for price	50 Reactions 50 Reactions

Formaldehyde Sodium Bisulfite
F691460	Toronto Research Chemicals	100g	EUR 63
Description: 870-72-4

Galactostatin Bisulfite Adduct
G156500	Toronto Research Chemicals	10mg	EUR 227

Acetone sodium bisulfite 97%
A04620	Pfaltz & Bauer	100G	EUR 314

Ammonium Bisulfite - In water
A634143	Toronto Research Chemicals	10ml	EUR 69
Description: 10192-30-0

Glutaraldehyde sodium bisulfite
RM9589-250G	EWC Diagnostics	1 unit	EUR 105.71
Description: Glutaraldehyde sodium bisulfite

BisulFlash DNA Bisulfite Conversion Mag-96 Kit
P-1050	EpiGentek	192 reactions	EUR 684.66

Chloroacetaldehyde Sodium Bisulfite
C382483	Toronto Research Chemicals	2.5g	EUR 87
Description: 13064-50-1

T 2588G Sodium Bisulfite adduct
T003103	Toronto Research Chemicals	10mg	EUR 1483

Adrenochrome Bisulfite Sodium Salt
A305255	Toronto Research Chemicals	100mg	EUR 181
Description: 5818-84-8

Menadione Sodium Bisulfite Hydrate
M218663	Toronto Research Chemicals	10g	EUR 65
Description: 217487-19-9

Acetone Sodium Bisulfite pure, 98%
62316	Sisco Laboratories	100 Gms	EUR 29.85
Description: Part B

Formaldehyde Sodium Bisulfite- 13CD2
F702573	Toronto Research Chemicals	10mg	EUR 1200

Methylamp Whole Cell Bisulfite Modification Kit
P-1016	EpiGentek	Ask for price Ask for price Ask for price	80 Samples 40 Samples 80 Samples

Ammonium bisulfite 25% aqueous soln.
A29947	Pfaltz & Bauer	250G	EUR 101

Menadione sodium bisulfite hydrate-13C6
M218105	Toronto Research Chemicals	50mg	EUR 15000

Glyoxal Sodium Bisulfite (contains oligomers)
G754513	Toronto Research Chemicals	10g	EUR 58
Description: 517-21-5

Formaldehyde/sodium bisulfite addition compound
F05023	Pfaltz & Bauer	500G	EUR 86

Ammonium bisulfite 45% w/v aqueous solution
A29946	Pfaltz & Bauer	250G	EUR 196.25

Glutaraldehyde sodium bisulfite addition compound
G02580	Pfaltz & Bauer	50G	EUR 206

3-Indoleacetaldehyde bisulfite addition compound
I01290	Pfaltz & Bauer	25MG	EUR 278.35

Sodium Bisulfite (mixture of NaHSO3 and Na2S2O5)
S614400	Toronto Research Chemicals	100g	EUR 80
Description: 7631-90-5

pOET Sequencing Primers
GWB-200100	GenWay Biotech	2 x 100 ul	Ask for price

SequaGel Sequencing System 1L Kit - 1KIT
NAT1136	Scientific Laboratory Supplies	1KIT	EUR 187.65

sequencing unit 33x45 cm
ESEQ1100-SYS	Consort	ea	EUR 1842

SequaGel Sequencing System 2.2L Kit - EACH
NAT1138	Scientific Laboratory Supplies	EACH	EUR 318.6

Galactostatin Bisulfite (5-Amino-5-deoxy-D-galactopyranose, Galactonojirimycin Bisulfite, 6-(Hydroxymethyl)-2,3,4,6-peperidinetetrol Hydrogensulfite)
MBS6043222-1mg	MyBiosource	1(mg	EUR 590

Galactostatin Bisulfite (5-Amino-5-deoxy-D-galactopyranose, Galactonojirimycin Bisulfite, 6-(Hydroxymethyl)-2,3,4,6-peperidinetetrol Hydrogensulfite)
MBS6043222-5x1mg	MyBiosource	5x1mg	EUR 2500

Sequencing Grade Chymotrypsin
RE013	SAB	100ug	EUR 309

Palmitaldehyde/Sodium Bisulfite Addition Compound _x000D__x000D_
P00520	Pfaltz & Bauer	5G	EUR 199.11

Trypin for Mass & Sequencing
T9600-025	GenDepot	25ug	EUR 174

Trypin for Mass & Sequencing
T9600-100	GenDepot	100ug	EUR 262.8

Trypin for Mass & Sequencing
T9600-112	GenDepot	12x100ug	EUR 1988.4

Trypin for Mass & Sequencing
T9600-400	GenDepot	4x100ug	EUR 781.2

sequencing system 20x50 cm
ESEQ1200-SYS	Consort	ea	EUR 1842

Sequencing Grade V8 Protease
RE014	SAB	50ug	EUR 209

5,6-Diamino-2,4-dihydroxypyrimidine-13C2 Bisulfite Salt
D416392	Toronto Research Chemicals	10mg	EUR 161

5-Hydroxyindole-3-acetaldehyde Sodium Bisulfite Adduct
H942933	Toronto Research Chemicals	500mg	EUR 3000

TNM Sequencing Primers for SE
TMS002-C1-1rxn	Vazyme	1 rxn	EUR 115

TNM Sequencing Primers for PE
TMS002-C2-1rxn	Vazyme	1 rxn	EUR 143.88

Chymotrypsin for Sequencing grade
C4001-010	GenDepot	4x25ug	EUR 375.6

Chymotrypsin for Sequencing grade
C4001-100	GenDepot	100ug	EUR 343.2

Sequencing Grade Modified Trypsin
RE012	SAB	100ug	EUR 229

Oligo, Sequencing Primer, SK (17mer)
MBS634188-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, SK (17mer)
MBS634188-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, KS (17mer)
MBS634192-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, KS (17mer)
MBS634192-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, SK (20mer)
MBS634219-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, SK (20mer)
MBS634219-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

Oligo, Sequencing Primer, T3 (17mer)
MBS634243-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, T3 (17mer)
MBS634243-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, T7 (17mer)
MBS634247-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, T7 (17mer)
MBS634247-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, KS (19mer)
MBS634259-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, KS (19mer)
MBS634259-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, T3 (20mer)
MBS634261-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, T3 (20mer)
MBS634261-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

DNA Library Prep Kit for IIlumina Sequencing
K1475-12	Biovision	12 Rxns	EUR 576

Sequencing Grade Carboxypeptidase B
RE011	SAB	100ug	EUR 99

EpiNext Post-Bisulfite DNA Library Preparation Kit (Illumina)
P-1055	EpiGentek	Ask for price Ask for price Ask for price	24 Reactions 12 Reactions 24 Reactions

PCR Clean Up for DNA Sequencing
BT5100	Bio Basic	100preps	EUR 114.82

PCR Clean Up for DNA Sequencing
BT5101	Bio Basic	1000Preps, 1000prep	EUR 553.3

ACES Sequencing Buffer 10X, pH 7.7
21420000-1	Glycomatrix	1 L	EUR 138.62

ACES Sequencing Buffer 10X, pH 7.7
21420000-2	Glycomatrix	4 L	EUR 456.17

TBE-Buffer 5X for DNA Sequencing
40121072-1	Glycomatrix	100 mL	EUR 26.54

TBE-Buffer 5X for DNA Sequencing
40121072-2	Glycomatrix	500 mL	EUR 44.84

TBE-Buffer 5X for DNA Sequencing
40121072-3	Glycomatrix	1 L	EUR 72.29

TBE-Buffer 5X for DNA Sequencing
40121072-4	Glycomatrix	4 L	EUR 192.14

Bovine Trypsin Purified Sequencing Grade
IBOTRYSEQ100UG	Innovative research	each	EUR 148
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade
IBOTRYSEQ1MG	Innovative research	each	EUR 765
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade
IBOTRYSEQ400UG	Innovative research	each	EUR 419
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade
MBS136298-01mg	MyBiosource	0.1mg	EUR 255

Bovine Trypsin Purified Sequencing Grade
MBS136298-04mg	MyBiosource	0.4mg	EUR 540

Bovine Trypsin Purified Sequencing Grade
MBS136298-1mg	MyBiosource	1mg	EUR 935

Bovine Trypsin Purified Sequencing Grade
MBS136298-5x1mg	MyBiosource	5x1mg	EUR 4010

MagaDye™ 4 Color Sanger Sequencing Terminator Kit
17068	AAT Bioquest	5 nmoles	EUR 2091

Oligo, Sequencing Primer, T7 Universal (20mer)
MBS634194-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, T7 Universal (20mer)
MBS634194-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

Oligo, Sequencing Primer, SP6 Promoter (19mer)
MBS634260-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, SP6 Promoter (19mer)
MBS634260-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Trifluoroacetic Acid (TFA) for HPLC & Sequencing, 99.9%
23947	Sisco Laboratories	10 x1 ml	EUR 165.03
Description: Part A

Oligo, Sequencing Primer, Lambda gt11, Reverse (24mer)
MBS634120-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, Lambda gt11, Reverse (24mer)
MBS634120-5x0033mg	MyBiosource	5x0.033mg	EUR 1245

Oligo, Sequencing Primer, Lambda gt10, Reverse (31mer)
MBS634167-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, Lambda gt10, Reverse (31mer)
MBS634167-5x0033mg	MyBiosource	5x0.033mg	EUR 1245

Oligo, Sequencing Primer, Lambda gt11, Forward (24mer)
MBS634252-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, Lambda gt11, Forward (24mer)
MBS634252-5x0033mg	MyBiosource	5x0.033mg	EUR 1245

Oligo, Sequencing Primer, Lambda gt10, Forward (27mer)
MBS634255-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, Lambda gt10, Forward (27mer)
MBS634255-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

Bovine Trypsin Purified Sequencing Grade Lyophilized
IBOTRYSEQLY100UG	Innovative research	each	EUR 130
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized
IBOTRYSEQLY1MG	Innovative research	each	EUR 640
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized
IBOTRYSEQLY400UG	Innovative research	each	EUR 344
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized
MBS136299-01mg	MyBiosource	0.1mg	EUR 235

Bovine Trypsin Purified Sequencing Grade Lyophilized
MBS136299-04mg	MyBiosource	0.4mg	EUR 465

Bovine Trypsin Purified Sequencing Grade Lyophilized
MBS136299-1mg	MyBiosource	1mg	EUR 795

Bovine Trypsin Purified Sequencing Grade Lyophilized
MBS136299-5x1mg	MyBiosource	5x1mg	EUR 3400

D-Mannojirimycin Bisulfite (5-Amino-5-deoxy-D-mannopyranose Hydrogensulfite)
MBS6055354-1mg	MyBiosource	1(mg	EUR 580

D-Mannojirimycin Bisulfite (5-Amino-5-deoxy-D-mannopyranose Hydrogensulfite)
MBS6055354-5x1mg	MyBiosource	5x1mg	EUR 2470

Oligo, Sequencing Primer, pBR322 (EcoR I), Clockwise (16mer)
MBS634158-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, pBR322 (EcoR I), Clockwise (16mer)
MBS634158-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

Oligo, Sequencing Primer, pBR322 (BamH I), Clockwise (20mer)
MBS634234-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, pBR322 (BamH I), Clockwise (20mer)
MBS634234-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

Oligo, Sequencing Primer, pBR322 (Pst I), Counterclockwise (16mer)
MBS634143-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, pBR322 (Pst I), Counterclockwise (16mer)
MBS634143-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

Oligo, Sequencing Primer, pBR322 (BamH I), Counterclockwise (16mer)
MBS634262-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, pBR322 (BamH I), Counterclockwise (16mer)
MBS634262-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

Oligo, Sequencing Primer, pBR322 (Hind III), Counterclockwise (16mer)
MBS634154-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, pBR322 (Hind III), Counterclockwise (16mer)
MBS634154-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

Discussion and conclusion: Validate any updates HMVV carried out in accordance with expert guidance. We turned the 8 × reduction in bioinformatics pipeline computing time to test our longest. Our molecular pathologists to interpret the test results of at least two days faster than was previously possible.