Background: To identify the relationship between somatic ataxia-telangiectasia mutated (ATM) mutation and improved radio-sensitivity, we retrospectively reviewed the next-generation sequencing data from patients diagnosed with glioma isocitrate dehydrogenase (IDH) -wildtype high quality.

Methods: We included 39 individuals (IDH) -wildtype high-grade glioma (diffuse astrocytoma n = 2, n = 10 anaplastic astrocytoma and glioblastoma n = 27) had no gross tumor resection and radiation therapy undergo the median total dose of 60 Gy in 30 fractions. ATM gene mutation status was obtained through generation sequencing using TruSight cancer panel 170. Tumor progression was defined by Response Assessment in Neuro-Oncology (Rano) criteria as well as neurological and clinical findings.
Results: Among the 39 samples, ATM mutations (mut ATM (+)) was detected in 26% of cases (n = 10).

No significant differences were observed in the patient or tumor characteristics. Among the 10 patients in the ATM mut (+), there are 6 patients with glioblastoma and four patients with anaplastic astrocytoma. Most mutations are missense mutations (n ​​= 8, 80%). With a median follow-up of 16.5 mo (interquartile range, 11.4 to 19.8), ATM mut (+) exhibited one year of control in the field of 100% compared with 44.1% in ATM mut (-) group (p = 0.002). There was no difference in the rate of out-of-field control or overall survival between the two groups (p = 0.861 and p = 0.247, respectively).

Conclusions: Our results indicate that ATM mutations may be involved in the increase in radio-sensitivity with good control in the field despite the aggressive nature of IDH-wildtype high-grade glioma. Further studies are needed to uncover the potential role of ATM as a candidate biomarker and therapeutic target in high-grade glioma.

Whole-genome sequencing and Mycobacterium tuberculosis: Challenges in sample preparation and analysis of sequencing data

The number of patients with tuberculosis (TB) caused by resistant strains are still alarming. Therefore, it is necessary to determine the resilience of faster and more accurate, than with the currently used phenotypic and genotypic methods.

In recent years, technological advances have been made and the method of whole genome sequencing (WGS) has been introduced as part of routine clinical laboratory diagnostics. Comparing various mycobacterial genome variation with reference genome leads to a consistent evaluation of molecular epidemiology and resistance of Mycobacterium tuberculosis (M. tuberculosis) to a variety of anti-TB drugs.

The quality of sequencing data obtained is closely related to the type of samples and the methods used for DNA extraction and sequencing library preparation. In addition, the correct interpretation of the results is also affected by bioinformatics data processing. A large number of bioinformatics pipeline that is currently available, the sensitivity varies due to the different size of the database containing the relevant mutation. This review focuses on the individual steps in the workflow including sequencing and factors that may affect the interpretation of the final results.

comparative analysis suggests that miR-375 and miR-7 expression was substantially higher in the case of the control of non-Nen Nen. Correlation analysis showed that NENs from diverse anatomical sites have convergent program miRNA expression, may reflect the morphological and functional similarities.

TBE-Buffer 5X for DNA Sequencing
40121072-3	Glycomatrix	1 L	EUR 72.29

TBE-Buffer 5X for DNA Sequencing
40121072-4	Glycomatrix	4 L	EUR 192.14

DNA Library Prep Kit for IIlumina Sequencing
K1475-12	Biovision	12 Rxns	EUR 576

Genorise® Customized PCR primers for cloning and sequencing
GR108023	Genorise Scientific	2 nmol	EUR 65

Trypin for Mass & Sequencing
T9600-025	GenDepot	25ug	EUR 174

Trypin for Mass & Sequencing
T9600-100	GenDepot	100ug	EUR 262.8

Trypin for Mass & Sequencing
T9600-112	GenDepot	12x100ug	EUR 1988.4

Trypin for Mass & Sequencing
T9600-400	GenDepot	4x100ug	EUR 781.2

Chymotrypsin for Sequencing grade
C4001-010	GenDepot	4x25ug	EUR 375.6

Chymotrypsin for Sequencing grade
C4001-100	GenDepot	100ug	EUR 343.2

TNM Sequencing Primers for SE
TMS002-C1-1rxn	Vazyme	1 rxn	EUR 115

TNM Sequencing Primers for PE
TMS002-C2-1rxn	Vazyme	1 rxn	EUR 143.88

Trifluoroacetic Acid (TFA) for HPLC & Sequencing, 99.9%
23947	Sisco Laboratories	10 x1 ml	EUR 165.03
Description: Part A

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK
AM-96-SEPTA-3100	CORNING	10/pk	EUR 639.6
Description: Sealing Products; Sealing mats - Axygen

pOET Sequencing Primers
GWB-200100	GenWay Biotech	2 x 100 ul	Ask for price

sequencing unit 33x45 cm
ESEQ1100-SYS	Consort	ea	EUR 1842

Sequencing Grade Chymotrypsin
RE013	SAB	100ug	EUR 309

PCR DNA CLEAN-UP KIT
IB47035	IBI Scientific	2 X 96 WELL FORMAT KIT	EUR 305.5

PCR DNA CLEAN-UP KIT
IB47040	IBI Scientific	4 X 96 WELL FORMAT KIT	EUR 608.35

PCR DNA CLEAN-UP KIT
IB47050	IBI Scientific	10 X 96 WELL FORMAT KIT	EUR 1425.45

sequencing system 20x50 cm
ESEQ1200-SYS	Consort	ea	EUR 1842

Sequencing Grade V8 Protease
RE014	SAB	50ug	EUR 209

Sequencing Grade Modified Trypsin
RE012	SAB	100ug	EUR 229

Oligo, Sequencing Primer, SK (17mer)
MBS634188-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, SK (17mer)
MBS634188-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, KS (17mer)
MBS634192-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, KS (17mer)
MBS634192-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, SK (20mer)
MBS634219-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, SK (20mer)
MBS634219-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

Oligo, Sequencing Primer, T3 (17mer)
MBS634243-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, T3 (17mer)
MBS634243-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, T7 (17mer)
MBS634247-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, T7 (17mer)
MBS634247-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, KS (19mer)
MBS634259-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, KS (19mer)
MBS634259-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, T3 (20mer)
MBS634261-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, T3 (20mer)
MBS634261-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

Sequencing Grade Carboxypeptidase B
RE011	SAB	100ug	EUR 99

ACES Sequencing Buffer 10X, pH 7.7
21420000-1	Glycomatrix	1 L	EUR 138.62

ACES Sequencing Buffer 10X, pH 7.7
21420000-2	Glycomatrix	4 L	EUR 456.17

SequaGel Sequencing System 1L Kit - 1KIT
NAT1136	Scientific Laboratory Supplies	1KIT	EUR 187.65

SequaGel Sequencing System 2.2L Kit - EACH
NAT1138	Scientific Laboratory Supplies	EACH	EUR 318.6

Oligo, Sequencing Primer, Lambda gt11, Forward (24mer)
MBS634252-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, Lambda gt11, Forward (24mer)
MBS634252-5x0033mg	MyBiosource	5x0.033mg	EUR 1245

Oligo, Sequencing Primer, Lambda gt10, Forward (27mer)
MBS634255-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, Lambda gt10, Forward (27mer)
MBS634255-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

Bovine Trypsin Purified Sequencing Grade
IBOTRYSEQ100UG	Innovative research	each	EUR 148
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade
IBOTRYSEQ1MG	Innovative research	each	EUR 765
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade
IBOTRYSEQ400UG	Innovative research	each	EUR 419
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade
MBS136298-01mg	MyBiosource	0.1mg	EUR 255

Bovine Trypsin Purified Sequencing Grade
MBS136298-04mg	MyBiosource	0.4mg	EUR 540

Bovine Trypsin Purified Sequencing Grade
MBS136298-1mg	MyBiosource	1mg	EUR 935

Bovine Trypsin Purified Sequencing Grade
MBS136298-5x1mg	MyBiosource	5x1mg	EUR 4010

EpiNext Bisulfite Sequencing Kit (Illumina)
P-1056	EpiGentek	Ask for price Ask for price Ask for price	24 Reactions 12 Reactions 24 Reactions

Oligo, Sequencing Primer, T7 Universal (20mer)
MBS634194-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, T7 Universal (20mer)
MBS634194-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

Oligo, Sequencing Primer, SP6 Promoter (19mer)
MBS634260-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, SP6 Promoter (19mer)
MBS634260-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, Lambda gt11, Reverse (24mer)
MBS634120-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, Lambda gt11, Reverse (24mer)
MBS634120-5x0033mg	MyBiosource	5x0.033mg	EUR 1245

Oligo, Sequencing Primer, Lambda gt10, Reverse (31mer)
MBS634167-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, Lambda gt10, Reverse (31mer)
MBS634167-5x0033mg	MyBiosource	5x0.033mg	EUR 1245

Bovine Trypsin Purified Sequencing Grade Lyophilized
IBOTRYSEQLY100UG	Innovative research	each	EUR 130
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized
IBOTRYSEQLY1MG	Innovative research	each	EUR 640
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized
IBOTRYSEQLY400UG	Innovative research	each	EUR 344
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized
MBS136299-01mg	MyBiosource	0.1mg	EUR 235

Bovine Trypsin Purified Sequencing Grade Lyophilized
MBS136299-04mg	MyBiosource	0.4mg	EUR 465

Bovine Trypsin Purified Sequencing Grade Lyophilized
MBS136299-1mg	MyBiosource	1mg	EUR 795

Bovine Trypsin Purified Sequencing Grade Lyophilized
MBS136299-5x1mg	MyBiosource	5x1mg	EUR 3400

PCR Clean-up Kit,
PC05	GeneOn	5 preps	Ask for price

PCR Clean-up Kit,
PC100	GeneOn	100 preps	EUR 156

PCR Clean-up Kit,
PC200	GeneOn	200 preps	EUR 226.8

PCR Clean-up Kit,
PC50	GeneOn	50 preps	EUR 112.8

MagaDye™ 4 Color Sanger Sequencing Terminator Kit
17068	AAT Bioquest	5 nmoles	EUR 2091

Oligo, Sequencing Primer, pBR322 (EcoR I), Clockwise (16mer)
MBS634158-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, pBR322 (EcoR I), Clockwise (16mer)
MBS634158-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

Oligo, Sequencing Primer, pBR322 (BamH I), Clockwise (20mer)
MBS634234-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, pBR322 (BamH I), Clockwise (20mer)
MBS634234-5x0033mg	MyBiosource	5x0.033mg	EUR 1240

GeneMAGNET PCR/ DNA CLEAN - UP Purification Kit
E3420-01	EURx	96preps	EUR 138.43

GeneMATRIX PCR/ DNA CLEAN - UP Purification Kit
E3520-01	EURx	50preps	EUR 50.14
Description: Kit for purification of PCR products / DNA after enzymatic reactions

GeneMATRIX PCR/ DNA CLEAN - UP Purification Kit
E3520-02	EURx	150preps	EUR 138.43
Description: Kit for purification of PCR products / DNA after enzymatic reactions

Oligo, Sequencing Primer, pBR322 (Pst I), Counterclockwise (16mer)
MBS634143-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, pBR322 (Pst I), Counterclockwise (16mer)
MBS634143-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

Oligo, Sequencing Primer, pBR322 (BamH I), Counterclockwise (16mer)
MBS634262-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, pBR322 (BamH I), Counterclockwise (16mer)
MBS634262-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

Oligo, Sequencing Primer, M13 (-20) Forward (17mer) [5'-GTA AAA CGA CGG CCA GT-3']
MBS634146-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, M13 (-20) Forward (17mer) [5'-GTA AAA CGA CGG CCA GT-3']
MBS634146-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

Oligo, Sequencing Primer, pBR322 (Hind III), Counterclockwise (16mer)
MBS634154-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, pBR322 (Hind III), Counterclockwise (16mer)
MBS634154-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

TuMinute PCR Clean-Up Kit
P-1005	EpiGentek	Ask for price Ask for price Ask for price	100 Samples 50 Samples 100 Samples

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade
IBOCHYTLCKSG100UG	Innovative research	each	EUR 297
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade
MBS136223-01mg	MyBiosource	0.1mg	EUR 400

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade
MBS136223-5x01mg	MyBiosource	5x0.1mg	EUR 1605

Oligo, Sequencing Primer, M13 Reverse (16mer) [5'-AAC AGC TAT GAC CAT G-3']
MBS634131-0033mg	MyBiosource	0.033mg	EUR 305

Oligo, Sequencing Primer, M13 Reverse (16mer) [5'-AAC AGC TAT GAC CAT G-3']
MBS634131-5x0033mg	MyBiosource	5x0.033mg	EUR 1230

Oligo, Sequencing Primer, M13 Reverse (19mer) [5'-GGA AAC AGC TAT GAC CAT G-3']
MBS634148-0033mg	MyBiosource	0.033mg	EUR 310

Oligo, Sequencing Primer, M13 Reverse (19mer) [5'-GGA AAC AGC TAT GAC CAT G-3']
MBS634148-5x0033mg	MyBiosource	5x0.033mg	EUR 1235

PCR Advanced Clean Up System
PF2001	Viogene	50 preps	EUR 104.58

PCR Advanced Clean Up System
PF2002	Viogene	250 preps	EUR 427.48

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified
IBCTCLOSGPF10UG	Innovative research	each	EUR 165
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified
MBS136171-01mg	MyBiosource	0.1mg	EUR 270

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified
MBS136171-5x001mg	MyBiosource	5x0.01mg	EUR 1035

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized
IBCTV8SEQLY250UG	Innovative research	each	EUR 784
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized
IBCTV8SEQLY50UG	Innovative research	each	EUR 304
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

PCR Clean-Up Mini Kit (50prep)
FAPCK-001	Favorgen	50 preps	EUR 144

PCR Clean-Up Mini Kit (200prep)
FAPCK-001-1	Favorgen	200 preps	EUR 202.8

PCR Clean-Up Mini Kit (300prep)
FAPCK-001-2	Favorgen	300 preps	EUR 226.8

PCR Clean-Up Mini Kit (50prep)
FAPCK_001	Favorgen	1 kit	EUR 16

PCR Clean-Up Mini Kit (200prep)
FAPCK_001-1	Favorgen	1 kit	EUR 54

PCR Clean-Up Mini Kit (300prep)
FAPCK_001-2	Favorgen	1 kit	EUR 69.5

DNA Clean-up Kit
MBS278006-200Preps	MyBiosource	200Preps	EUR 250

DNA Clean-up Kit
MBS278006-50Preps	MyBiosource	50Preps	EUR 180

PCR Clean-Up & Gel Extraction Kit
PDC01-0100	Bio-Helix	100 rxns	EUR 55

PCR Clean-Up & Gel Extraction Kit
MBS402028-100Reactions	MyBiosource	100Reactions	EUR 240

PCR Clean-Up & Gel Extraction Kit
MBS402028-4Reactions	MyBiosource	4Reactions	EUR 185

MicroElute PCR Clean Up Kit (50prep)
FAMPK-001	Favorgen	50 preps	EUR 151.2

MicroElute PCR Clean Up Kit (200prep)
FAMPK-001-1	Favorgen	200 preps	EUR 224.4

MicroElute PCR Clean Up Kit (100prep) (blister packing for columns)
FAMPK_001-1B	Favorgen	1 kit	EUR 56

MicroElute PCR Clean Up Kit (50prep) (blister packing for columns)
FAMPK_001B	Favorgen	1 kit	EUR 28

AxyPrep Mag PCR clean up -50mL - EACH
AXY1046	Scientific Laboratory Supplies	EACH	EUR 1934.55

GF-1 PCR Clean-up Kit, 50 preps
GF-PC-050	Vivantis	each	Ask for price

GF-1 PCR Clean-up Kit, 100 preps
GF-PC-100	Vivantis	each	Ask for price

GF-1 PCR Clean-up Kit, 200 preps
GF-PC-200	Vivantis	each	Ask for price

96-well PCR Clean-Up Kit (1 prep)
FACKE-96001	Favorgen	1 prep	EUR 189.6

96-well PCR Clean-Up Kit (2 prep)
FACKE-96002	Favorgen	2 preps	EUR 255.6

96-well PCR Clean-Up Kit (4 prep)
FACKE-96004	Favorgen	4 preps	EUR 380.4

96-well PCR Clean-Up Kit (1 prep)
FACKE_96001	Favorgen	1 kit	EUR 45

96-well PCR Clean-Up Kit (2 prep)
FACKE_96002	Favorgen	1 kit	EUR 87.5

96-well PCR Clean-Up Kit (4 prep)
FACKE_96004	Favorgen	1 kit	EUR 168

AxyPrep Mag PCR clean up Kit-1mL - EACH
AXY1040	Scientific Laboratory Supplies	EACH	EUR 102.6

AxyPrep Mag PCR clean up Kit-5mL - EACH
AXY1044	Scientific Laboratory Supplies	EACH	EUR 410.4

Column-Pure Gel and PCR Clean-Up Kit
D516	ABM	100 Preparations	EUR 165

Eppendorf DNA LoBind tubes 0.5ml PCR clean - PK250
E0030108035	Scientific Laboratory Supplies	PK250	EUR 35.1

Eppendorf DNA LoBind tubes 1.5ml PCR clean - PK250
E0030108051	Scientific Laboratory Supplies	PK250	EUR 20.25

Eppendorf DNA LoBind tubes 2.0ml PCR clean - PK250
E0030108078	Scientific Laboratory Supplies	PK250	EUR 22.95

AXYPREP MAG PCR CLEAN UP KIT- 5 ML - 110 PREPS
MAG-PCR-CL-5	CORNING	1/pk	EUR 175.2
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AXYPREP MAG PCR CLEAN UP KIT- 50 ML - 1110 PREPS
MAG-PCR-CL-50	CORNING	1/pk	EUR 694.8
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

DNA Advanced DNA Clean Up and Concentrator System
DC1001	Viogene	50 preps	EUR 104.58

DNA Advanced DNA Clean Up and Concentrator System
DC1002	Viogene	250 preps	EUR 427.48

AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE)
MAG-PCR-CL-1	CORNING	1/pk	EUR 69.6
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

96-well GE/PCR Clean Up Purification Kit (1 plates)
FAPKE-96001	Favorgen	1 prep	EUR 175.2

96-well GE/PCR Clean Up Purification Kit (2 plates)
FAPKE-96002	Favorgen	2 preps	EUR 225.6

96-well GE/PCR Clean Up Purification Kit (4 plates)
FAPKE-96004	Favorgen	4 preps	EUR 328.8

96-well GE/PCR Clean Up Purification Kit (1 plates)
FAPKE_96001	Favorgen	1 kit	EUR 36

96-well GE/PCR Clean Up Purification Kit (2 plates)
FAPKE_96002	Favorgen	1 kit	EUR 68.5

96-well GE/PCR Clean Up Purification Kit (4 plates)
FAPKE_96004	Favorgen	1 kit	EUR 134.5

FavorPrep Genomic DNA Clean-Up Kit (50prep)
FAGDC-001	Favorgen	50 preps	EUR 154.8

FavorPrep Genomic DNA Clean-Up Kit (200prep)
FAGDC-001-1	Favorgen	200 preps	EUR 244.8

FavorPrep Genomic DNA Clean-Up Kit (50prep)
FAGDC_001	Favorgen	1 kit	EUR 22.5

FavorPrep Genomic DNA Clean-Up Kit (200prep)
FAGDC_001-1	Favorgen	1 kit	EUR 81

GeneMATRIX SHORT DNA CLEAN - UP Purification Kit
E3515-01	EURx	25preps	EUR 46.87
Description: Kit for purification of short single-stranded and double-stranded DNA fragments after enzymatic reactions

GeneMATRIX SHORT DNA CLEAN - UP Purification Kit
E3515-02	EURx	100preps	EUR 167.86
Description: Kit for purification of short single-stranded and double-stranded DNA fragments after enzymatic reactions

Eppendorf tubes 5mL screw cap DNA LoBind PCR clean - PK200
E0030122348	Scientific Laboratory Supplies	PK200	EUR 86.4

GF-1 96-well PCR Clean-up Kit, 96 x 5 plates
GF-96-C05	Vivantis	each	Ask for price

GF-1 96-well PCR Clean-up Kit, 96 x 10 plates
GF-96-C10	Vivantis	each	Ask for price

PAGE Clean Up Kit
06441-50	NACALAI TESQUE	1KIT	EUR 269.5

Tubes 5mL screw cap PCR clean - PK200
E0030122313	Scientific Laboratory Supplies	PK200	EUR 78.3

Micro test tubes 3810X PCR clean - PK1000
E0030125215	Scientific Laboratory Supplies	PK1000	EUR 55.35

Using machine learning approach, we identified 17 miRNAs to distinguish 15 Nen type of pathological and then build a classifier multilayer, correctly identified 217 (98%) of the 221 samples and overturning a histological diagnosis.