Background: To identify the relationship between somatic ataxia-telangiectasia mutated (ATM) mutation and improved radio-sensitivity, we retrospectively reviewed the next-generation sequencing data from patients diagnosed with glioma isocitrate dehydrogenase (IDH) -wildtype high quality.

Methods: We included 39 individuals (IDH) -wildtype high-grade glioma (diffuse astrocytoma n = 2, n = 10 anaplastic astrocytoma and glioblastoma n = 27) had no gross tumor resection and radiation therapy undergo the median total dose of 60 Gy in 30 fractions. ATM gene mutation status was obtained through generation sequencing using TruSight cancer panel 170. Tumor progression was defined by Response Assessment in Neuro-Oncology (Rano) criteria as well as neurological and clinical findings.
Results: Among the 39 samples, ATM mutations (mut ATM (+)) was detected in 26% of cases (n = 10).

No significant differences were observed in the patient or tumor characteristics. Among the 10 patients in the ATM mut (+), there are 6 patients with glioblastoma and four patients with anaplastic astrocytoma. Most mutations are missense mutations (n ​​= 8, 80%). With a median follow-up of 16.5 mo (interquartile range, 11.4 to 19.8), ATM mut (+) exhibited one year of control in the field of 100% compared with 44.1% in ATM mut (-) group (p = 0.002). There was no difference in the rate of out-of-field control or overall survival between the two groups (p = 0.861 and p = 0.247, respectively).

Conclusions: Our results indicate that ATM mutations may be involved in the increase in radio-sensitivity with good control in the field despite the aggressive nature of IDH-wildtype high-grade glioma. Further studies are needed to uncover the potential role of ATM as a candidate biomarker and therapeutic target in high-grade glioma.

Whole-genome sequencing and Mycobacterium tuberculosis: Challenges in sample preparation and analysis of sequencing data

The number of patients with tuberculosis (TB) caused by resistant strains are still alarming. Therefore, it is necessary to determine the resilience of faster and more accurate, than with the currently used phenotypic and genotypic methods.

In recent years, technological advances have been made and the method of whole genome sequencing (WGS) has been introduced as part of routine clinical laboratory diagnostics. Comparing various mycobacterial genome variation with reference genome leads to a consistent evaluation of molecular epidemiology and resistance of Mycobacterium tuberculosis (M. tuberculosis) to a variety of anti-TB drugs.

The quality of sequencing data obtained is closely related to the type of samples and the methods used for DNA extraction and sequencing library preparation. In addition, the correct interpretation of the results is also affected by bioinformatics data processing. A large number of bioinformatics pipeline that is currently available, the sensitivity varies due to the different size of the database containing the relevant mutation. This review focuses on the individual steps in the workflow including sequencing and factors that may affect the interpretation of the final results.

comparative analysis suggests that miR-375 and miR-7 expression was substantially higher in the case of the control of non-Nen Nen. Correlation analysis showed that NENs from diverse anatomical sites have convergent program miRNA expression, may reflect the morphological and functional similarities.

PCR Clean-up Kit,
PC200	GeneOn	200 preps	EUR 226.8

PCR Clean-up Kit,
PC50	GeneOn	50 preps	EUR 112.8

TuMinute PCR Clean-Up Kit
P-1005-1	EpiGentek	50 Samples	EUR 90.2

TuMinute PCR Clean-Up Kit
P-1005-2	EpiGentek	100 Samples	EUR 171.6

TuMinute PCR Clean-Up Kit
P-1005	EpiGentek	EUR 303.60 EUR 90.20 EUR 171.60	100 Samples 50 Samples 100 Samples

MicroElute PCR Clean Up Kit (50prep)
FAMPK-001	Favorgen	50 preps	EUR 151.2

MicroElute PCR Clean Up Kit (200prep)
FAMPK-001-1	Favorgen	200 preps	EUR 224.4

PCR Clean-Up Mini Kit (50prep)
FAPCK-001	Favorgen	50 preps	EUR 144

PCR Clean-Up Mini Kit (200prep)
FAPCK-001-1	Favorgen	200 preps	EUR 202.8

PCR Clean-Up Mini Kit (300prep)
FAPCK-001-2	Favorgen	300 preps	EUR 226.8

AxyPrep Mag PCR clean up -50mL
AXY1046	Scientific Laboratory Supplies	EACH	EUR 1511.64

AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE)
MAG-PCR-CL-1	CORNING	1/pk	EUR 69.6
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AXYPREP MAG PCR CLEAN UP KIT- 5 ML - 110 PREPS
MAG-PCR-CL-5	CORNING	1/pk	EUR 175.2
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AXYPREP MAG PCR CLEAN UP KIT- 50 ML - 1110 PREPS
MAG-PCR-CL-50	CORNING	1/pk	EUR 694.8
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

FavorPrep Genomic DNA Clean-Up Kit (50prep)
FAGDC-001	Favorgen	50 preps	EUR 154.8

FavorPrep Genomic DNA Clean-Up Kit (200prep)
FAGDC-001-1	Favorgen	200 preps	EUR 244.8

AxyPrep Mag PCR clean up Kit-1mL
AXY1040	Scientific Laboratory Supplies	EACH	EUR 79.8

AxyPrep Mag PCR clean up Kit-5mL
AXY1044	Scientific Laboratory Supplies	EACH	EUR 320.34

96-well PCR Clean-Up Kit (1 prep)
FACKE-96001	Favorgen	1 prep	EUR 189.6

96-well PCR Clean-Up Kit (2 prep)
FACKE-96002	Favorgen	2 preps	EUR 255.6

96-well PCR Clean-Up Kit (4 prep)
FACKE-96004	Favorgen	4 preps	EUR 380.4

Eppendorf DNA LoBind tubes 0.5ml PCR clean
E0030108035	Scientific Laboratory Supplies	PK250	EUR 27.36

Eppendorf DNA LoBind tubes 1.5ml PCR clean
E0030108051	Scientific Laboratory Supplies	PK250	EUR 15.07

Eppendorf DNA LoBind tubes 2.0ml PCR clean
E0030108078	Scientific Laboratory Supplies	PK250	EUR 18.02

DNA Library Prep Kit for IIlumina Sequencing
K1475-12	Biovision	12 Rxns	EUR 576

96-well GE/PCR Clean Up Purification Kit (1 plates)
FAPKE-96001	Favorgen	1 prep	EUR 175.2

96-well GE/PCR Clean Up Purification Kit (2 plates)
FAPKE-96002	Favorgen	2 preps	EUR 225.6

96-well GE/PCR Clean Up Purification Kit (4 plates)
FAPKE-96004	Favorgen	4 preps	EUR 328.8

Gel Permeation Clean Up Calibration Standard
GPC-CAL-91	Scientific Laboratory Supplies	5ML	EUR 92.34

VAHTS DNA Clean Beads
N411-01	Vazyme	5 ml	EUR 259.2

VAHTS DNA Clean Beads
N411-02	Vazyme	60 ml	EUR 634.8

VAHTS DNA Clean Beads
N411-03	Vazyme	450 ml	EUR 3055.2

Eppendorf tubes 5mL screw cap DNA LoBind PCR clean
E0030122348	Scientific Laboratory Supplies	PK200	EUR 68.4

Genorise® Customized PCR primers for cloning and sequencing
GR108023	Genorise Scientific	2 nmol	EUR 78

Chymotrypsin for Sequencing grade
C4001-010	GenDepot	4x25ug	EUR 375.6

Chymotrypsin for Sequencing grade
C4001-100	GenDepot	100ug	EUR 343.2

Trypin for Mass & Sequencing
T9600-025	GenDepot	25ug	EUR 174

Trypin for Mass & Sequencing
T9600-100	GenDepot	100ug	EUR 262.8

Trypin for Mass & Sequencing
T9600-112	GenDepot	12x100ug	EUR 1988.4

Trypin for Mass & Sequencing
T9600-400	GenDepot	4x100ug	EUR 781.2

Zenoquick DNA Clean & Concentrator-5
Z2001-050	GenDepot	50 prpe	EUR 216

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 50
GR101080	Genorise Scientific	50	EUR 177.6

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 100
GR101080-100	Genorise Scientific	100	EUR 320.4

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 200
GR101081	Genorise Scientific	200	EUR 572.4

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 500
GR101081-500	Genorise Scientific	500	EUR 1288.8

EpiQuik DNA Clean and Concentrator Kit
P-1068-050	EpiGentek	50 preparations	EUR 88

EpiQuik DNA Clean and Concentrator Kit
P-1068-100	EpiGentek	100 preparations	EUR 167.2

EpiQuik DNA Clean and Concentrator Kit
P-1068	EpiGentek	EUR 88.00 EUR 167.20	50 preparations 100 preparations

epTIPS Reloads 0.1-10ul PCR-clean
E0030073754	Scientific Laboratory Supplies	PK960	EUR 80.94

epTIPS Reloads 0.1-20ul PCR-clean
E0030073770	Scientific Laboratory Supplies	PK960	EUR 83.22

epTIPS Reloads 0.5-20ul PCR-clean
E0030073797	Scientific Laboratory Supplies	PK960	EUR 77.52

epTIPS Reloads 2-200ul PCR-clean
E0030073819	Scientific Laboratory Supplies	PK960	EUR 72.96

epTIPS Reloads 20-30ul PCR-clean
E0030073835	Scientific Laboratory Supplies	PK960	EUR 72.96

epTIPS Reloads 50-1000ul PCR-clean
E0030073851	Scientific Laboratory Supplies	PK960	EUR 72.96

epTIPS Reloads 50-1250ul PCR-clean
E0030073878	Scientific Laboratory Supplies	PK960	EUR 88.92

epTIPS Reloads 250-2500ul PCR-clean
E0030073894	Scientific Laboratory Supplies	PK480	EUR 58.94

Tubes 5mL screw cap PCR clean
E0030122313	Scientific Laboratory Supplies	PK200	EUR 61.56

Micro test tubes 3810X PCR clean
E0030125215	Scientific Laboratory Supplies	PK1000	EUR 43.32

100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP	CORNING	1/pk	EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB	CORNING	1/pk	EUR 84
Description: Bioscience Mol Bio; DNA Ladder

Eppendorf Protein LoBind tubes 0.5ml PCR clean
E0030108094	Scientific Laboratory Supplies	PK100	EUR 14.58

Eppendorf Protein LoBind tubes 1.5ml PCR clean
E0030108116	Scientific Laboratory Supplies	PK100	EUR 10.93

Eppendorf Protein LoBind tubes 2.0ml PCR clean
E0030108132	Scientific Laboratory Supplies	PK100	EUR 17.4

PCR Tubes (50)
PCR-50	BioAssay Systems	50	EUR 15
Description: To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes.

Automatic FillDrain for Clean Steam for Rodwell autoclaves
AUT1351	Scientific Laboratory Supplies	EACH	EUR 2211.6

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK
AM-96-SEPTA-3100	CORNING	10/pk	EUR 639.6
Description: Sealing Products; Sealing mats - Axygen

Tips PCR Clean 50uL 103 mm dark green 10x96
E0030073649	Scientific Laboratory Supplies	EACH	EUR 90.06

epT.I.P.S. G Reloads 384 PCR clean 0.1 - 20uL 42mm
E0030076001	Scientific Laboratory Supplies	PK3840	EUR 531.24

epT.I.P.S. G Reload 384 PCR clean 0.5 - 100uL 52.8mm
E0030076010	Scientific Laboratory Supplies	PK3840	EUR 531.24

epT.I.P.S. Reload 384 IVD PCR clean 0.1 - 20uL 42mm
E0030076060	Scientific Laboratory Supplies	PK3840	EUR 539.22

epT.I.P.S. Reload 384 IVD PCR clean 0.5 - 100uL 53mm
E0030076079	Scientific Laboratory Supplies	PK3840	EUR 539.22

Combitips Adv. PCR Clean 1.0ml 4 packs of 25
E0030089790	Scientific Laboratory Supplies	PK100	EUR 123.12

Combitips Adv. PCR Clean 2.5ml 4 packs of 25
E0030089804	Scientific Laboratory Supplies	PK100	EUR 123.12

Combitips Adv. PCR Clean 10ml 4 packs of 25
E0030089820	Scientific Laboratory Supplies	PK100	EUR 123.12

Combitips Adv. PCR Clean 25ml 4 packs of 25
E0030089839	Scientific Laboratory Supplies	PK100	EUR 144.78

Combitips Adv. PCR Clean 50ml 4 packs of 25
E0030089847	Scientific Laboratory Supplies	PK100	EUR 156.18

Eppendorf Conical Tubes 25mL with snap cap PCR clean
E0030118413	Scientific Laboratory Supplies	PK200	EUR 64.98

Eppendorf tubes 5mL screw cap protein LoBind PCR clean
E0030122356	Scientific Laboratory Supplies	PK200	EUR 92.34

Eppendorf Conical Tubes 25mL with screw cap PCR clean
E0030122429	Scientific Laboratory Supplies	PK200	EUR 69.54

Eppendorf Safe-Lock micro test tubes 0.5ml PCR clean
E0030123301	Scientific Laboratory Supplies	PK500	EUR 50.62

Eppendorf Safe-Lock micro test tubes 1.5ml PCR clean
E0030123328	Scientific Laboratory Supplies	PK1000	EUR 50.62

Eppendorf Safe-Lock micro test tubes 2.0ml PCR clean
E0030123344	Scientific Laboratory Supplies	PK1000	EUR 69.54

sequencing unit 33x45 cm
ESEQ1100-SYS	Consort	ea	EUR 1842

sequencing system 20x50 cm
ESEQ1200-SYS	Consort	ea	EUR 1842

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173L	MiTeGen	960 reactions x 20 µl	EUR 789.36
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173S	MiTeGen	192 reactions x 20 µl	EUR 197.34
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

Taq DNA Polymerase (PCR)
G008	ABM	5000 U (1.0 ml)	EUR 246

Taq DNA Polymerase (PCR)
G009	ABM	1000 U (200 ul)	EUR 104.4

HotStart DNA Polymerase (PCR)
G011	ABM	250 U (50 ul)	EUR 98.4

HotStart DNA Polymerase (PCR)
G039	ABM	1000 U (200 ul)	EUR 189.6

Taq DNA Polymerase (PCR)
G126	ABM	10000 U (2 x 1.0 ml)	EUR 370.8

TaqFast DNA Polymerase (PCR)
G277	ABM	250 U (50 ul)	EUR 87.6

TaqFast DNA Polymerase (PCR)
G278	ABM	1000 U (200 ul)	EUR 160.8

Bloodirect DNA Polymerase (PCR)
G462	ABM	100 U (100 ul)	EUR 73.2

Bloodirect DNA Polymerase (PCR)
G463	ABM	400 U (400 ul)	EUR 104.4

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C	CORNING	10/pk	EUR 200.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL PCR MICROPLATES FOR MEGA BASE
PCR-96-MB-C	CORNING	10/pk	EUR 174
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D	Bioingentech	50T	EUR 543.6

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D	Bioingentech	100T	EUR 686.4

CONNECTION KIT FOR START-UP
STARTKIT1	Scientific Laboratory Supplies	EACH	EUR 229.14

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D	Bioingentech	50T	EUR 543.6

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D	Bioingentech	100T	EUR 686.4

SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service
M-PCR-378	MiTeGen	500 µl	EUR 63.63
Description: SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service

ep LoRetention PCR clean 0.1-10ul 10 x 96 reloads
E0030072006	Scientific Laboratory Supplies	PK960	EUR 88.92

ep LoRetention PCR clean 0.5-20ul 10 x 96 reloads
E0030072014	Scientific Laboratory Supplies	PK960	EUR 85.5

ep LoRetention PCR clean 2-200ul 10 x 96 reloads
E0030072022	Scientific Laboratory Supplies	PK960	EUR 80.94

ep LoRetention PCR clean 50-1000ul 10 x 96 reloads
E0030072030	Scientific Laboratory Supplies	PK960	EUR 80.94

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR
PCR-2CP-RT-C	CORNING	125/pk	EUR 267.6
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

lyophilised real-time PCR Master Mix for dual labeled probes
M-PCR-156L	MiTeGen	960 reactions x 20 µl	EUR 900.68
Description: lyophilised real-time PCR Master Mix for dual labeled probes

lyophilised real-time PCR Master Mix for dual labeled probes
M-PCR-156S	MiTeGen	192 reactions x 20 µl	EUR 225.3
Description: lyophilised real-time PCR Master Mix for dual labeled probes

Lancer Clean Detergent
CLE1136	Scientific Laboratory Supplies	10L	EUR 72.96

SequaGel Sequencing System 1L Kit
NAT1136	Scientific Laboratory Supplies	1KIT	EUR 127.68

SequaGel Sequencing System 2.2L Kit
NAT1138	Scientific Laboratory Supplies	EACH	EUR 217.74

EpiNext Bisulfite Sequencing Kit (Illumina)
P-1056-12	EpiGentek	12 Reactions	EUR 625.9

EpiNext Bisulfite Sequencing Kit (Illumina)
P-1056-24	EpiGentek	24 Reactions	EUR 1225.4

EpiNext Bisulfite Sequencing Kit (Illumina)
P-1056	EpiGentek	EUR 1798.26 EUR 625.90 EUR 1225.40	24 Reactions 12 Reactions 24 Reactions

ranavirus PCR kit
PCR-V337-48D	Bioingentech	50T	EUR 543.6

ranavirus PCR kit
PCR-V337-96D	Bioingentech	100T	EUR 686.4

Ruminococcus PCR kit
PCR-V619-48D	Bioingentech	50T	EUR 510.96
Description: An conventional PCR kit for detection of Ruminococcus

Ruminococcus PCR kit
PCR-V619-96D	Bioingentech	100T	EUR 625.8
Description: An conventional PCR kit for detection of Ruminococcus

Bifidobacterium PCR kit
PCR-V621-48D	Bioingentech	50T	EUR 510.96
Description: An conventional PCR kit for detection of Bifidobacterium

Bifidobacterium PCR kit
PCR-V621-96D	Bioingentech	100T	EUR 625.8
Description: An conventional PCR kit for detection of Bifidobacterium

Halobacillus PCR kit
PCR-A635-48D	Bioingentech	50T	EUR 543.6

Halobacillus PCR kit
PCR-A635-96D	Bioingentech	100T	EUR 686.4

Enterovirus PCR kit
PCR-H433-48R	Bioingentech	50T	EUR 987.6

Enterovirus PCR kit
PCR-H433-96R	Bioingentech	100T	EUR 1030.8

Mengovirus PCR kit
PCR-H453-48R	Bioingentech	50T	EUR 987.6

Mengovirus PCR kit
PCR-H453-96R	Bioingentech	100T	EUR 1335.6

Astrovirus PCR kit
PCR-H491-48R	Bioingentech	50T	EUR 987.6

Astrovirus PCR kit
PCR-H491-96R	Bioingentech	100T	EUR 1335.6

Calicivirus PCR kit
PCR-H493-48R	Bioingentech	50T	EUR 987.6

Calicivirus PCR kit
PCR-H493-96R	Bioingentech	100T	EUR 1335.6

Adenovirus PCR kit
PCR-H501-48D	Bioingentech	50T	EUR 543.6

Adenovirus PCR kit
PCR-H501-96D	Bioingentech	100T	EUR 686.4

Polyomavirus PCR kit
PCR-H515-48D	Bioingentech	50T	EUR 543.6

Polyomavirus PCR kit
PCR-H515-96D	Bioingentech	100T	EUR 686.4

Ruminococcus PCR kit
PCR-H619-48D	Bioingentech	48T	EUR 543.6

Ruminococcus PCR kit
PCR-H619-96D	Bioingentech	96T	EUR 686.4

Bifidobacterium PCR kit
PCR-H621-48D	Bioingentech	48T	EUR 543.6

Bifidobacterium PCR kit
PCR-H621-96D	Bioingentech	96T	EUR 686.4

Sapovirus PCR kit
PCR-H630-48R	Bioingentech	50T	EUR 987.6

Sapovirus PCR kit
PCR-H630-96R	Bioingentech	100T	EUR 1335.6

Parechovirus PCR kit
PCR-H642-48R	Bioingentech	50T	EUR 813.6

Parechovirus PCR kit
PCR-H642-96R	Bioingentech	100T	EUR 1335.6

Metapneumovirus PCR kit
PCR-H643-48R	Bioingentech	50T	EUR 987.6

Metapneumovirus PCR kit
PCR-H643-96R	Bioingentech	100T	EUR 1335.6

Entamoeba PCR kit
PCR-H683-48D	Bioingentech	50T	EUR 543.6

Entamoeba PCR kit
PCR-H683-96D	Bioingentech	100T	EUR 686.4

Acanthamoeba PCR kit
PCR-H684-48D	Bioingentech	50T	EUR 543.6

Acanthamoeba PCR kit
PCR-H684-96D	Bioingentech	100T	EUR 686.4

Echovirus PCR kit
PCR-H722-48D	Bioingentech	50T	EUR 543.6

Echovirus PCR kit
PCR-H722-96D	Bioingentech	100T	EUR 686.4

PCR DNA Extraction and Purification
EP10013	Neuromics	100 Tests	EUR 356.4

Taq Plus DNA Polymerase (PCR)
G012	ABM	250 U (50 ul)	EUR 87.6

Taq Plus DNA Polymerase (PCR)
G040	ABM	1000 U (200 ul)	EUR 127.2

Precision™ DNA Polymerase (PCR)
G078	ABM	500 U (100 ul)	EUR 133.2

Bestaq™ DNA Polymerase (PCR)
G456	ABM	250 U (50 ul)	EUR 104.4

Bestaq™ DNA Polymerase (PCR)
G457	ABM	1000 U (200 ul)	EUR 189.6

Using machine learning approach, we identified 17 miRNAs to distinguish 15 Nen type of pathological and then build a classifier multilayer, correctly identified 217 (98%) of the 221 samples and overturning a histological diagnosis.