ATM mutations improve radio-sensitivity in wild-type isocitrate dehydrogenase-associated high-grade glioma: retrospective analysis using next-generation sequencing data

ATM mutations improve radio-sensitivity in wild-type isocitrate dehydrogenase-associated high-grade glioma: retrospective analysis using next-generation sequencing data

Background: To identify the relationship between somatic ataxia-telangiectasia mutated (ATM) mutation and improved radio-sensitivity, we retrospectively reviewed the next-generation sequencing data from patients diagnosed with glioma isocitrate dehydrogenase (IDH) -wildtype high quality.


Methods: We included 39 individuals (IDH) -wildtype high-grade glioma (diffuse astrocytoma n = 2, n = 10 anaplastic astrocytoma and glioblastoma n = 27) had no gross tumor resection and radiation therapy undergo the median total dose of 60 Gy in 30 fractions. ATM gene mutation status was obtained through generation sequencing using TruSight cancer panel 170. Tumor progression was defined by Response Assessment in Neuro-Oncology (Rano) criteria as well as neurological and clinical findings.
Results: Among the 39 samples, ATM mutations (mut ATM (+)) was detected in 26% of cases (n = 10).

No significant differences were observed in the patient or tumor characteristics. Among the 10 patients in the ATM mut (+), there are 6 patients with glioblastoma and four patients with anaplastic astrocytoma. Most mutations are missense mutations (n ​​= 8, 80%). With a median follow-up of 16.5 mo (interquartile range, 11.4 to 19.8), ATM mut (+) exhibited one year of control in the field of 100% compared with 44.1% in ATM mut (-) group (p = 0.002). There was no difference in the rate of out-of-field control or overall survival between the two groups (p = 0.861 and p = 0.247, respectively).


Conclusions: Our results indicate that ATM mutations may be involved in the increase in radio-sensitivity with good control in the field despite the aggressive nature of IDH-wildtype high-grade glioma. Further studies are needed to uncover the potential role of ATM as a candidate biomarker and therapeutic target in high-grade glioma.

 ATM mutations improve radio-sensitivity in wild-type isocitrate dehydrogenase-associated high-grade glioma: retrospective analysis using next-generation sequencing data
ATM mutations improve radio-sensitivity in wild-type isocitrate dehydrogenase-associated high-grade glioma: retrospective analysis using next-generation sequencing data

Whole-genome sequencing and Mycobacterium tuberculosis: Challenges in sample preparation and analysis of sequencing data

The number of patients with tuberculosis (TB) caused by resistant strains are still alarming. Therefore, it is necessary to determine the resilience of faster and more accurate, than with the currently used phenotypic and genotypic methods.

In recent years, technological advances have been made and the method of whole genome sequencing (WGS) has been introduced as part of routine clinical laboratory diagnostics. Comparing various mycobacterial genome variation with reference genome leads to a consistent evaluation of molecular epidemiology and resistance of Mycobacterium tuberculosis (M. tuberculosis) to a variety of anti-TB drugs.

The quality of sequencing data obtained is closely related to the type of samples and the methods used for DNA extraction and sequencing library preparation. In addition, the correct interpretation of the results is also affected by bioinformatics data processing. A large number of bioinformatics pipeline that is currently available, the sensitivity varies due to the different size of the database containing the relevant mutation. This review focuses on the individual steps in the workflow including sequencing and factors that may affect the interpretation of the final results.


comparative analysis suggests that miR-375 and miR-7 expression was substantially higher in the case of the control of non-Nen Nen. Correlation analysis showed that NENs from diverse anatomical sites have convergent program miRNA expression, may reflect the morphological and functional similarities.

PCR Clean-up Kit,

PC05 5 preps Ask for price

PCR Clean-up Kit,

PC100 100 preps
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PCR Clean-up Kit,

PC50 50 preps
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TuMinute PCR Clean-Up Kit

P-1005 100 Samples
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Description: reagents widely cited

MicroElute PCR Clean Up Kit (50prep)

FAMPK-001 50 preps
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MicroElute PCR Clean Up Kit (200prep)

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PCR Clean-Up Mini Kit (50prep)

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AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE)

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FavorPrep Genomic DNA Clean-Up Kit (50prep)

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96-well PCR Clean-Up Kit (1 prep)

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96-well GE/PCR Clean Up Purification Kit (1 plates)

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VAHTS DNA Clean Beads

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5-min. Genomic DNA Prep for PCR

P930-100 NULL
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5-min. Genomic DNA Prep for PCR

P930-5 NULL
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5-min. Genomic DNA Prep for PCR

P930-500 NULL
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sequencing unit 33x45 cm

ESEQ1100-SYS ea
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SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

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96 WELL CLEAR PCR PLATE FOR ABI

PCR-96-AB-C 10/pk
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G008 5000 U (1.0 ml)
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G126 10000 U (2 x 1.0 ml)
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G277 250 U (50 ul)
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MULTIPLEX KIT PCR MASTITIS PCR kit

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MULTIPLEX KIT PCR MASTITIS PCR kit

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MULTIPLEX KIT PCR Babesia & Theileria PCR kit

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N412-01 5 ml
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N412-02 40 ml
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SequaGel Sequencing System 1L Kit

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Halobacillus PCR kit

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Enterovirus PCR kit

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PCR-H501-48D 50T
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PCR-H515-48D 50T
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PCR-H515-96D 100T
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PCR-H630-48R 50T
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Description: An conventional PCR kit for detection of Sapovirus

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Description: An conventional PCR kit for detection of Parechovirus

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PCR-H643-48R 50T
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Description: An conventional PCR kit for detection of Metapneumovirus

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PCR-H643-96R 100T
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Entamoeba PCR kit

PCR-H683-48D 50T
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PCR-H684-48D 50T
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Acanthamoeba PCR kit

PCR-H684-96D 100T
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Description: An conventional PCR kit for detection of Acanthamoeba

Echovirus PCR kit

PCR-H722-48D 50T
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Description: An conventional PCR kit for detection of Echovirus

Echovirus PCR kit

PCR-H722-96D 100T
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Description: An conventional PCR kit for detection of Echovirus

ranavirus PCR kit

PCR-V337-48D 50T
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Description: An conventional PCR kit for detection of ranavirus

ranavirus PCR kit

PCR-V337-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of ranavirus

Ruminococcus PCR kit

PCR-V619-48D 50T
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Description: An conventional PCR kit for detection of Ruminococcus

Ruminococcus PCR kit

PCR-V619-96D 100T
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Description: An conventional PCR kit for detection of Ruminococcus

Bifidobacterium PCR kit

PCR-V621-48D 50T
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Description: An conventional PCR kit for detection of Bifidobacterium

Bifidobacterium PCR kit

PCR-V621-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Bifidobacterium

Hantavirus PCR kit

PCR-VH373-48R 50T
EUR 524.40
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Description: An conventional PCR kit for detection of Hantavirus

Hantavirus PCR kit

PCR-VH373-96R 100T
EUR 669.40
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Description: An conventional PCR kit for detection of Hantavirus

Taq Plus DNA Polymerase (PCR)

G012 250 U (50 ul)
EUR 73.00

Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
EUR 106.00

Precision™ DNA Polymerase (PCR)

G078 500 U (100 ul)
EUR 111.00

Bestaq™ DNA Polymerase (PCR)

G456 250 U (50 ul)
EUR 87.00

Bestaq™ DNA Polymerase (PCR)

G457 1000 U (200 ul)
EUR 158.00

Long-Range DNA Polymerase (PCR)

G460 250 U (50 ul)
EUR 78.00

Long-Range DNA Polymerase (PCR)

G461 1000 U (200 ul)
EUR 153.00

PCR DNA Extraction and Purification

EP10013 100 Tests
EUR 297.00

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007
  • EUR 217.00
  • EUR 356.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Rack for up to 3 microplates

BV1010-MP 1 PC
EUR 95.45
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Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 72.00

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 193.00

48 WELL PCR MICROPLATE

PCR-48-C 10/pk
EUR 102.00
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Microthrix parvicella PCR kit

PCR-A575-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Microthrix parvicella

Microthrix parvicella PCR kit

PCR-A575-96D 100T
EUR 521.50
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Yersinia pseudotuberculosis PCR kit

PCR-H190-48D 50T
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Yersinia pseudotuberculosis PCR kit

PCR-H190-96D 100T
EUR 521.50
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Neisseria meningitidis PCR kit

PCR-H421-48D 50T
EUR 425.80
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Neisseria meningitidis PCR kit

PCR-H421-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Neisseria meningitidis

Neisseria sp. PCR kit

PCR-H422-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Neisseria sp.

Neisseria sp. PCR kit

PCR-H422-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Neisseria sp.

Haemophilus influenzae PCR kit

PCR-H423-48D 50T
EUR 425.80
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Haemophilus influenzae PCR kit

PCR-H423-96D 100T
EUR 521.50
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Trypanosoma cruzi PCR kit

PCR-H425-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Trypanosoma cruzi

Trypanosoma cruzi PCR kit

PCR-H425-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Trypanosoma cruzi

Cronobacter sakazakii PCR kit

PCR-H431-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Cronobacter sakazakii

Cronobacter sakazakii PCR kit

PCR-H431-96D 100T
EUR 521.50
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Clostridium botulinum PCR kit

PCR-H432-48D 50T
EUR 425.80
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Clostridium botulinum PCR kit

PCR-H432-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Clostridium botulinum

Virus Zika PCR kit

PCR-H434-48R 50T
EUR 524.40
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Description: An conventional PCR kit for detection of Virus Zika

Virus Zika PCR kit

PCR-H434-96R 100T
EUR 669.40
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Description: An conventional PCR kit for detection of Virus Zika

Corynebacterium diphtheriae PCR kit

PCR-H435-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Corynebacterium diphtheriae

Corynebacterium diphtheriae PCR kit

PCR-H435-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Corynebacterium diphtheriae

Legionella pneumophila PCR kit

PCR-H436-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Legionella pneumophila

Legionella pneumophila PCR kit

PCR-H436-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Legionella pneumophila

Bordetella pertussis PCR kit

PCR-H437-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Bordetella pertussis

Bordetella pertussis PCR kit

PCR-H437-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Bordetella pertussis

Bordetella parapertussis PCR kit

PCR-H438-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Bordetella parapertussis

Bordetella parapertussis PCR kit

PCR-H438-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Bordetella parapertussis

Chlamydia pneumoniae PCR kit

PCR-H439-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Chlamydia pneumoniae

Chlamydia pneumoniae PCR kit

PCR-H439-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Chlamydia pneumoniae

Pneumocystis jiroveci PCR kit

PCR-H440-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Pneumocystis jiroveci

Pneumocystis jiroveci PCR kit

PCR-H440-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Pneumocystis jiroveci

Actinobacillus actinomycetemcomitans PCR kit

PCR-H446-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Actinobacillus actinomycetemcomitans

Actinobacillus actinomycetemcomitans PCR kit

PCR-H446-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Actinobacillus actinomycetemcomitans

Bacteroides fragilis PCR kit

PCR-H447-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Bacteroides fragilis

Bacteroides fragilis PCR kit

PCR-H447-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Bacteroides fragilis

Candida sp. PCR kit

PCR-H448-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Candida sp.

Candida sp. PCR kit

PCR-H448-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Candida sp.

Enterococcus sp. PCR kit

PCR-H449-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Enterococcus sp.

Enterococcus sp. PCR kit

PCR-H449-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Enterococcus sp.

Neisseria gonorrhoeae PCR kit

PCR-H450-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Neisseria gonorrhoeae

Neisseria gonorrhoeae PCR kit

PCR-H450-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Neisseria gonorrhoeae

Dengue virus PCR kit

PCR-H451-48R 50T
EUR 524.40
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Description: An conventional PCR kit for detection of Dengue virus

Dengue virus PCR kit

PCR-H451-96R 100T
EUR 669.40
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Description: An conventional PCR kit for detection of Dengue virus

Chlamydia trachomatis PCR kit

PCR-H452-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Chlamydia trachomatis

Chlamydia trachomatis PCR kit

PCR-H452-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Chlamydia trachomatis

Human papillomavirus PCR kit

PCR-H454-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Human papillomavirus

Human papillomavirus PCR kit

PCR-H454-96D 100T
EUR 521.50
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Description: An conventional PCR kit for detection of Human papillomavirus

Parvovirus В19 PCR kit

PCR-H455-48D 50T
EUR 425.80
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Description: An conventional PCR kit for detection of Parvovirus В19

Parvovirus В19 PCR kit

PCR-H455-96D 100T
EUR 521.50
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Parvovirus В19

Treponema pallidum PCR kit

PCR-H456-48D 50T
EUR 425.80
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Treponema pallidum

Treponema pallidum PCR kit

PCR-H456-96D 100T
EUR 521.50
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Treponema pallidum

Trichomona vaginalis PCR kit

PCR-H457-48D 50T
EUR 425.80
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Trichomona vaginalis

Trichomona vaginalis PCR kit

PCR-H457-96D 100T
EUR 521.50
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Trichomona vaginalis

Using machine learning approach, we identified 17 miRNAs to distinguish 15 Nen type of pathological and then build a classifier multilayer, correctly identified 217 (98%) of the 221 samples and overturning a histological diagnosis.

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