Background: To identify the relationship between somatic ataxia-telangiectasia mutated (ATM) mutation and improved radio-sensitivity, we retrospectively reviewed the next-generation sequencing data from patients diagnosed with glioma isocitrate dehydrogenase (IDH) -wildtype high quality.

Methods: We included 39 individuals (IDH) -wildtype high-grade glioma (diffuse astrocytoma n = 2, n = 10 anaplastic astrocytoma and glioblastoma n = 27) had no gross tumor resection and radiation therapy undergo the median total dose of 60 Gy in 30 fractions. ATM gene mutation status was obtained through generation sequencing using TruSight cancer panel 170. Tumor progression was defined by Response Assessment in Neuro-Oncology (Rano) criteria as well as neurological and clinical findings.
Results: Among the 39 samples, ATM mutations (mut ATM (+)) was detected in 26% of cases (n = 10).

No significant differences were observed in the patient or tumor characteristics. Among the 10 patients in the ATM mut (+), there are 6 patients with glioblastoma and four patients with anaplastic astrocytoma. Most mutations are missense mutations (n ​​= 8, 80%). With a median follow-up of 16.5 mo (interquartile range, 11.4 to 19.8), ATM mut (+) exhibited one year of control in the field of 100% compared with 44.1% in ATM mut (-) group (p = 0.002). There was no difference in the rate of out-of-field control or overall survival between the two groups (p = 0.861 and p = 0.247, respectively).

Conclusions: Our results indicate that ATM mutations may be involved in the increase in radio-sensitivity with good control in the field despite the aggressive nature of IDH-wildtype high-grade glioma. Further studies are needed to uncover the potential role of ATM as a candidate biomarker and therapeutic target in high-grade glioma.

Whole-genome sequencing and Mycobacterium tuberculosis: Challenges in sample preparation and analysis of sequencing data

The number of patients with tuberculosis (TB) caused by resistant strains are still alarming. Therefore, it is necessary to determine the resilience of faster and more accurate, than with the currently used phenotypic and genotypic methods.

In recent years, technological advances have been made and the method of whole genome sequencing (WGS) has been introduced as part of routine clinical laboratory diagnostics. Comparing various mycobacterial genome variation with reference genome leads to a consistent evaluation of molecular epidemiology and resistance of Mycobacterium tuberculosis (M. tuberculosis) to a variety of anti-TB drugs.

The quality of sequencing data obtained is closely related to the type of samples and the methods used for DNA extraction and sequencing library preparation. In addition, the correct interpretation of the results is also affected by bioinformatics data processing. A large number of bioinformatics pipeline that is currently available, the sensitivity varies due to the different size of the database containing the relevant mutation. This review focuses on the individual steps in the workflow including sequencing and factors that may affect the interpretation of the final results.

comparative analysis suggests that miR-375 and miR-7 expression was substantially higher in the case of the control of non-Nen Nen. Correlation analysis showed that NENs from diverse anatomical sites have convergent program miRNA expression, may reflect the morphological and functional similarities.

PCR Clean-up Kit,
PC50	GeneOn	50 preps	EUR 94

TuMinute PCR Clean-Up Kit
P-1005	EpiGentek	100 Samples	EUR 253
Description: reagents widely cited

MicroElute PCR Clean Up Kit (50prep)
FAMPK-001	Favorgen	50 preps	EUR 126

MicroElute PCR Clean Up Kit (200prep)
FAMPK-001-1	Favorgen	200 preps	EUR 187

PCR Clean-Up Mini Kit (50prep)
FAPCK-001	Favorgen	50 preps	EUR 120

PCR Clean-Up Mini Kit (200prep)
FAPCK-001-1	Favorgen	200 preps	EUR 169

PCR Clean-Up Mini Kit (300prep)
FAPCK-001-2	Favorgen	300 preps	EUR 189

AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE)
MAG-PCR-CL-1	CORNING	1/pk	EUR 58
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AXYPREP MAG PCR CLEAN UP KIT- 5 ML - 110 PREPS
MAG-PCR-CL-5	CORNING	1/pk	EUR 146
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AXYPREP MAG PCR CLEAN UP KIT- 50 ML - 1110 PREPS
MAG-PCR-CL-50	CORNING	1/pk	EUR 579
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

FavorPrep Genomic DNA Clean-Up Kit (50prep)
FAGDC-001	Favorgen	50 preps	EUR 129

FavorPrep Genomic DNA Clean-Up Kit (200prep)
FAGDC-001-1	Favorgen	200 preps	EUR 204

96-well PCR Clean-Up Kit (1 prep)
FACKE-96001	Favorgen	1 prep	EUR 158

96-well PCR Clean-Up Kit (2 prep)
FACKE-96002	Favorgen	2 preps	EUR 213

96-well PCR Clean-Up Kit (4 prep)
FACKE-96004	Favorgen	4 preps	EUR 317

96-well GE/PCR Clean Up Purification Kit (1 plates)
FAPKE-96001	Favorgen	1 prep	EUR 146

96-well GE/PCR Clean Up Purification Kit (2 plates)
FAPKE-96002	Favorgen	2 preps	EUR 188

96-well GE/PCR Clean Up Purification Kit (4 plates)
FAPKE-96004	Favorgen	4 preps	EUR 274

DNA Library Prep Kit for IIlumina Sequencing
K1475-12	Biovision	12 Rxns	EUR 480

VAHTS DNA Clean Beads
N411-01	Vazyme	5 ml	EUR 216

VAHTS DNA Clean Beads
N411-02	Vazyme	60 ml	EUR 529

VAHTS DNA Clean Beads
N411-03	Vazyme	450 ml	EUR 2546

Zenoquick DNA Clean & Concentrator-5
Z2001-050	GenDepot	50 prpe	EUR 180

Trypin for Mass & Sequencing
T9600-025	GenDepot	25ug	EUR 145

Trypin for Mass & Sequencing
T9600-100	GenDepot	100ug	EUR 219

Trypin for Mass & Sequencing
T9600-112	GenDepot	12x100ug	EUR 1657

Trypin for Mass & Sequencing
T9600-400	GenDepot	4x100ug	EUR 651

Chymotrypsin for Sequencing grade
C4001-010	GenDepot	4x25ug	EUR 313

Chymotrypsin for Sequencing grade
C4001-100	GenDepot	100ug	EUR 286

100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP	CORNING	1/pk	EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB	CORNING	1/pk	EUR 70
Description: Bioscience Mol Bio; DNA Ladder

5-min. Genomic DNA Prep for PCR
P930-100	101Bio	-	Ask for price

5-min. Genomic DNA Prep for PCR
P930-5	101Bio	-	Ask for price

5-min. Genomic DNA Prep for PCR
P930-500	101Bio	-	Ask for price

sequencing unit 33x45 cm
ESEQ1100-SYS	Consort	ea	EUR 1535

sequencing system 20x50 cm
ESEQ1200-SYS	Consort	ea	EUR 1535

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK
AM-96-SEPTA-3100	CORNING	10/pk	EUR 533
Description: Sealing Products; Sealing mats - Axygen

Taq DNA Polymerase (PCR)
G008	ABM	5000 U (1.0 ml)	EUR 205

Taq DNA Polymerase (PCR)
G009	ABM	1000 U (200 ul)	EUR 87

HotStart DNA Polymerase (PCR)
G011	ABM	250 U (50 ul)	EUR 82

HotStart DNA Polymerase (PCR)
G039	ABM	1000 U (200 ul)	EUR 158

Taq DNA Polymerase (PCR)
G126	ABM	10000 U (2 x 1.0 ml)	EUR 309

TaqFast DNA Polymerase (PCR)
G277	ABM	250 U (50 ul)	EUR 73

TaqFast DNA Polymerase (PCR)
G278	ABM	1000 U (200 ul)	EUR 134

Bloodirect DNA Polymerase (PCR)
G462	ABM	100 U (100 ul)	EUR 61

Bloodirect DNA Polymerase (PCR)
G463	ABM	400 U (400 ul)	EUR 87

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C	CORNING	10/pk	EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL PCR MICROPLATES FOR MEGA BASE
PCR-96-MB-C	CORNING	10/pk	EUR 145
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D	Bioingentech	50T	EUR 453

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D	Bioingentech	100T	EUR 572

VAHTS RNA Clean Beads
N412-01	Vazyme	5 ml	EUR 239

VAHTS RNA Clean Beads
N412-02	Vazyme	40 ml	EUR 413

VAHTS RNA Clean Beads
N412-03	Vazyme	450 ml	EUR 4050

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D	Bioingentech	50T	EUR 453

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D	Bioingentech	100T	EUR 572

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR
PCR-2CP-RT-C	CORNING	125/pk	EUR 223
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

SequaGel Sequencing System 1L Kit
NAT1136	National Diagnostics	1KIT	EUR 143

SequaGel Sequencing System 2.2L Kit
NAT1138	National Diagnostics	EACH	EUR 211

Halobacillus PCR kit
PCR-A635-48D	Bioingentech	50T	EUR 453

Halobacillus PCR kit
PCR-A635-96D	Bioingentech	100T	EUR 572

Enterovirus PCR kit
PCR-H433-48R	Bioingentech	50T	EUR 823

Enterovirus PCR kit
PCR-H433-96R	Bioingentech	100T	EUR 859

Mengovirus PCR kit
PCR-H453-48R	Bioingentech	50T	EUR 823

Mengovirus PCR kit
PCR-H453-96R	Bioingentech	100T	EUR 1113

Astrovirus PCR kit
PCR-H491-48R	Bioingentech	50T	EUR 823

Astrovirus PCR kit
PCR-H491-96R	Bioingentech	100T	EUR 1113

Calicivirus PCR kit
PCR-H493-48R	Bioingentech	50T	EUR 823

Calicivirus PCR kit
PCR-H493-96R	Bioingentech	100T	EUR 1113

Adenovirus PCR kit
PCR-H501-48D	Bioingentech	50T	EUR 453

Adenovirus PCR kit
PCR-H501-96D	Bioingentech	100T	EUR 572

Polyomavirus PCR kit
PCR-H515-48D	Bioingentech	50T	EUR 453

Polyomavirus PCR kit
PCR-H515-96D	Bioingentech	100T	EUR 572

Ruminococcus PCR kit
PCR-H619-48D	Bioingentech	48T	EUR 453

Ruminococcus PCR kit
PCR-H619-96D	Bioingentech	96T	EUR 572

Bifidobacterium PCR kit
PCR-H621-48D	Bioingentech	48T	EUR 453

Bifidobacterium PCR kit
PCR-H621-96D	Bioingentech	96T	EUR 572

Sapovirus PCR kit
PCR-H630-48R	Bioingentech	50T	EUR 823

Sapovirus PCR kit
PCR-H630-96R	Bioingentech	100T	EUR 1113

Parechovirus PCR kit
PCR-H642-48R	Bioingentech	50T	EUR 678

Parechovirus PCR kit
PCR-H642-96R	Bioingentech	100T	EUR 1113

Metapneumovirus PCR kit
PCR-H643-48R	Bioingentech	50T	EUR 823

Metapneumovirus PCR kit
PCR-H643-96R	Bioingentech	100T	EUR 1113

Entamoeba PCR kit
PCR-H683-48D	Bioingentech	50T	EUR 453

Entamoeba PCR kit
PCR-H683-96D	Bioingentech	100T	EUR 572

Acanthamoeba PCR kit
PCR-H684-48D	Bioingentech	50T	EUR 453

Acanthamoeba PCR kit
PCR-H684-96D	Bioingentech	100T	EUR 572

Echovirus PCR kit
PCR-H722-48D	Bioingentech	50T	EUR 453

Echovirus PCR kit
PCR-H722-96D	Bioingentech	100T	EUR 572

ranavirus PCR kit
PCR-V337-48D	Bioingentech	50T	EUR 453

ranavirus PCR kit
PCR-V337-96D	Bioingentech	100T	EUR 572

Ruminococcus PCR kit
PCR-V619-48D	Bioingentech	50T	EUR 425.8
Description: An conventional PCR kit for detection of Ruminococcus

Ruminococcus PCR kit
PCR-V619-96D	Bioingentech	100T	EUR 521.5
Description: An conventional PCR kit for detection of Ruminococcus

Bifidobacterium PCR kit
PCR-V621-48D	Bioingentech	50T	EUR 425.8
Description: An conventional PCR kit for detection of Bifidobacterium

Bifidobacterium PCR kit
PCR-V621-96D	Bioingentech	100T	EUR 521.5
Description: An conventional PCR kit for detection of Bifidobacterium

Rack for up to 3 microplates
BV1010-MP	BenchMark	1 PC	EUR 95.45

Taq Plus DNA Polymerase (PCR)
G012	ABM	250 U (50 ul)	EUR 73

Taq Plus DNA Polymerase (PCR)
G040	ABM	1000 U (200 ul)	EUR 106

Precision™ DNA Polymerase (PCR)
G078	ABM	500 U (100 ul)	EUR 111

Bestaq™ DNA Polymerase (PCR)
G456	ABM	250 U (50 ul)	EUR 87

Bestaq™ DNA Polymerase (PCR)
G457	ABM	1000 U (200 ul)	EUR 158

Long-Range DNA Polymerase (PCR)
G460	ABM	250 U (50 ul)	EUR 78

Long-Range DNA Polymerase (PCR)
G461	ABM	1000 U (200 ul)	EUR 153

PCR DNA Extraction and Purification
EP10013	Neuromics	100 Tests	EUR 297

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
20-abx098007	Abbexa	EUR 217.00 EUR 356.00	1 ml 5 ml

Universal platform with adjustable bars, 2 x 1000mL flasks or 4 x 500mL flasks or 4 x 250mL flasks for UniPact?
ACT-USI-UP	ACTGene		EUR 532

48 WELL PCR MICROPLATE
PCR-48-C	CORNING	10/pk	EUR 102
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Microthrix parvicella PCR kit
PCR-A575-48D	Bioingentech	50T	EUR 453

Microthrix parvicella PCR kit
PCR-A575-96D	Bioingentech	100T	EUR 572

Yersinia pseudotuberculosis PCR kit
PCR-H190-48D	Bioingentech	50T	EUR 453

Yersinia pseudotuberculosis PCR kit
PCR-H190-96D	Bioingentech	100T	EUR 572

Neisseria meningitidis PCR kit
PCR-H421-48D	Bioingentech	50T	EUR 453

Neisseria meningitidis PCR kit
PCR-H421-96D	Bioingentech	100T	EUR 572

Neisseria sp. PCR kit
PCR-H422-48D	Bioingentech	50T	EUR 453

Neisseria sp. PCR kit
PCR-H422-96D	Bioingentech	100T	EUR 572

Haemophilus influenzae PCR kit
PCR-H423-48D	Bioingentech	50T	EUR 453

Haemophilus influenzae PCR kit
PCR-H423-96D	Bioingentech	100T	EUR 572

Trypanosoma cruzi PCR kit
PCR-H425-48D	Bioingentech	50T	EUR 453

Trypanosoma cruzi PCR kit
PCR-H425-96D	Bioingentech	100T	EUR 572

Cronobacter sakazakii PCR kit
PCR-H431-48D	Bioingentech	50T	EUR 453

Cronobacter sakazakii PCR kit
PCR-H431-96D	Bioingentech	100T	EUR 572

Clostridium botulinum PCR kit
PCR-H432-48D	Bioingentech	50T	EUR 453

Clostridium botulinum PCR kit
PCR-H432-96D	Bioingentech	100T	EUR 572

Virus Zika PCR kit
PCR-H434-48R	Bioingentech	50T	EUR 823

Virus Zika PCR kit
PCR-H434-96R	Bioingentech	100T	EUR 1113

Corynebacterium diphtheriae PCR kit
PCR-H435-48D	Bioingentech	50T	EUR 453

Corynebacterium diphtheriae PCR kit
PCR-H435-96D	Bioingentech	100T	EUR 572

Legionella pneumophila PCR kit
PCR-H436-48D	Bioingentech	50T	EUR 453

Legionella pneumophila PCR kit
PCR-H436-96D	Bioingentech	100T	EUR 572

Bordetella pertussis PCR kit
PCR-H437-48D	Bioingentech	50T	EUR 453

Bordetella pertussis PCR kit
PCR-H437-96D	Bioingentech	100T	EUR 572

Bordetella parapertussis PCR kit
PCR-H438-48D	Bioingentech	50T	EUR 453

Bordetella parapertussis PCR kit
PCR-H438-96D	Bioingentech	100T	EUR 572

Chlamydia pneumoniae PCR kit
PCR-H439-48D	Bioingentech	50T	EUR 453

Chlamydia pneumoniae PCR kit
PCR-H439-96D	Bioingentech	100T	EUR 572

Pneumocystis jiroveci PCR kit
PCR-H440-48D	Bioingentech	50T	EUR 453

Pneumocystis jiroveci PCR kit
PCR-H440-96D	Bioingentech	100T	EUR 572

Actinobacillus actinomycetemcomitans PCR kit
PCR-H446-48D	Bioingentech	50T	EUR 453

Actinobacillus actinomycetemcomitans PCR kit
PCR-H446-96D	Bioingentech	100T	EUR 572

Bacteroides fragilis PCR kit
PCR-H447-48D	Bioingentech	50T	EUR 453

Bacteroides fragilis PCR kit
PCR-H447-96D	Bioingentech	100T	EUR 572

Candida sp. PCR kit
PCR-H448-48D	Bioingentech	50T	EUR 453

Candida sp. PCR kit
PCR-H448-96D	Bioingentech	100T	EUR 572

Enterococcus sp. PCR kit
PCR-H449-48D	Bioingentech	50T	EUR 453

Enterococcus sp. PCR kit
PCR-H449-96D	Bioingentech	100T	EUR 572

Neisseria gonorrhoeae PCR kit
PCR-H450-48D	Bioingentech	50T	EUR 453

Neisseria gonorrhoeae PCR kit
PCR-H450-96D	Bioingentech	100T	EUR 572

Dengue virus PCR kit
PCR-H451-48R	Bioingentech	50T	EUR 823

Dengue virus PCR kit
PCR-H451-96R	Bioingentech	100T	EUR 1113

Chlamydia trachomatis PCR kit
PCR-H452-48D	Bioingentech	50T	EUR 453

Chlamydia trachomatis PCR kit
PCR-H452-96D	Bioingentech	100T	EUR 572

Human papillomavirus PCR kit
PCR-H454-48D	Bioingentech	50T	EUR 453

Human papillomavirus PCR kit
PCR-H454-96D	Bioingentech	100T	EUR 572

Parvovirus В19 PCR kit
PCR-H455-48D	Bioingentech	50T	EUR 453

Parvovirus В19 PCR kit
PCR-H455-96D	Bioingentech	100T	EUR 572

Treponema pallidum PCR kit
PCR-H456-48D	Bioingentech	50T	EUR 453

Treponema pallidum PCR kit
PCR-H456-96D	Bioingentech	100T	EUR 572

Trichomona vaginalis PCR kit
PCR-H457-48D	Bioingentech	50T	EUR 453

Trichomona vaginalis PCR kit
PCR-H457-96D	Bioingentech	100T	EUR 572

Using machine learning approach, we identified 17 miRNAs to distinguish 15 Nen type of pathological and then build a classifier multilayer, correctly identified 217 (98%) of the 221 samples and overturning a histological diagnosis.